TOMM22 Antibody (1C9-2)

Novus Biologicals | Catalog # NB120-10436

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Key Product Details

Species Reactivity

Human, Mouse (Negative), Primate, Rat (Negative)

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1C9-2
View all TOMM22 Primary Antibodies »

Product Specifications

Immunogen

Membrane fraction from Vero (monkey kidney-derived) cells. Uniprot accession no. Q9NS69.

Reactivity Notes

Cross-reacts with Human and Monkey.Not yet tested in other species. Should not react with rodent.

Localization

Mitochondrial

Specificity

TOMM22 (1C9-2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TOMM22 Antibody (1C9-2)

Western Blot: TOMM22 Antibody (1C9-2) [NB120-10436]

Western Blot: TOMM22 Antibody (1C9-2) [NB120-10436]

Western Blot: TOMM22 Antibody (1C9-2) [NB120-10436] - Whole extract of cultured Human lymphoma Raji cells were separated on SDS-PAGE and probed with Anti-TOM22 antibody, Mouse Monoclonal, Clone: 1C9-2. The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat.Lanes:1. 0.5 ug/mL antibody2. 1 ug/mL antibody3. Negative Control

Applications for TOMM22 Antibody (1C9-2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

0.5 - 1.0 ug/ml
Application Notes
ICC: Use at an assay dependent dilution. Western Blot: Use at a concentration of 0.5 - 1.0 ug/ml. Detects a band of approximately 22 kDa. Optimal dilutions/concentrations should be determined by the end user.

Reviewed Applications

Read 1 review rated 5 using NB120-10436 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4) and 1.0% BSA

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TOMM22

Tom22 has a negatively charged N-terminal region exposed to the cytosol, a putative transmembrane region, and a C-terminal intermembrane space region with little negative charge. The following functions can be assigned to the three domains of Tom22: the cytosolic N-terminal domain plays a dual role, specifically important for presequence binding; the intermembrane space domain provides a trans binding site for presequences, and the single membrane anchor of Tom22 is crucial for the integrity of the GIP (general import gene) complex. In the absence of this membrane anchor, the GIP complex dissociates into small core complexes containing a dimer of Tom40 and the three small Toms. A preprotein is stably arrested and accumulated in the GIP complex by Tom40 and Tom22.4 Such a 100 kDa core complex probably contains a single channel that retains the basic channel properties but is already open in the absence of preproteins. In contrast, in the presence of Tom22, the wild-type GIP complex contains tightly regulated channels (probably three channels). Tom22 apparently represents a component of the machinery that controls the gate. The cytosolic domains of Tom22 and Tom20 are believed to form the major part of a cis site, which mediates the import of all preproteins known to use the general import machinery of mitochondria. The preprotein is then routed through the Tom complex translocation channel and transferred to a trans site on the intermembrane space (IMS) side of the outer membrane. The inter-membrane space exposed segment of Tom40 and the C-terminal tail of Tom22 may contribute to the trans-site. Matrix-targeted proteins are further transferred to the matrix through Product Information import machinery in the inner membrane. The TOM complex of mammalian mitochondria resembles the fungal Tom complex, but is distinct from the plant TOM system. Thus, while unique components of the mammalian mitochondrial import system have been identified (e.g. TOM34 and metaxin), Tom22, and Tom37 have not been identified in plant mitochondria.

Alternate Names

1C9-2, hTom22, mitochondrial import receptor subunit TOM22 homolog, mitochondrial import receptor Tom22, MST065, TOM22MSTP065, Translocase of outer membrane 22 kDa subunit homolog, translocase of outer mitochondrial membrane 22 homolog (yeast)

Entrez Gene IDs

56993 (Human)

Gene Symbol

TOMM22

Additional TOMM22 Products

Product Documents for TOMM22 Antibody (1C9-2)

Certificate of Analysis

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Product Specific Notices for TOMM22 Antibody (1C9-2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TOMM22 Antibody (1C9-2) (1)

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  • TOMM22 Antibody (1C9-2)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Small intestine tissue
    Species: Human
    Verified Customer | Posted 12/27/2021
    Small intestine tissue. The antibody dilution for TOMM22 antibody was 1:500.
    TOMM22 Antibody (1C9-2) NB120-10436

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