TOP2B Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89527

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat, Insect - Drosophila

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TOP2B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEITAMKADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOP2B Antibody - BSA Free

TOP2B Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: TOP2B Antibody - BSA Free [NBP1-89527]

Immunocytochemistry/ Immunofluorescence: TOP2B Antibody - BSA Free [NBP1-89527]

Staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527]

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527]

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human pancreas shows weak to moderate nuclear positivity in islets of Langerhans and exocrine glandular cells.
Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527]

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527]

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527]

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527]

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human gastrointestinal shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527]

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527]

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human kidney shows moderate nuclear positivity in cells in glomeruli and tubules.
Simple Western: TOP2B Antibody [NBP1-89527]

Simple Western: TOP2B Antibody [NBP1-89527]

Simple Western: TOP2B Antibody [NBP1-89527] - Simple Western lane view shows a specific band for TOP2B in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.
Simple Western: TOP2B Antibody [NBP1-89527]

Simple Western: TOP2B Antibody [NBP1-89527]

Simple Western: TOP2B Antibody [NBP1-89527] - Electropherogram image(s) of corresponding Simple Western lane view. TOP2B antibody was used at 1:100 dilution on RT-4 lysate(s).
TOP2B Antibody - BSA Free Western Blot: TOP2B Antibody - BSA Free [NBP1-89527]

Western Blot: TOP2B Antibody - BSA Free [NBP1-89527]

Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TOP2B antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
TOP2B Antibody - BSA Free Western Blot: TOP2B Antibody - BSA Free [NBP1-89527]

Western Blot: TOP2B Antibody - BSA Free [NBP1-89527]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for TOP2B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

1:100

Western Blot

0.04-0.4 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4, separated by Size, antibody dilution of 1:100, apparent MW was 199 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-89527 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TOP2B

Topo II Beta antibody plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells. Further, it is implicated in drug resistance of tumor cells.

Long Name

DNA Topoisomerase II beta

Alternate Names

antigen MLAA-44, DNA topoisomerase 2-beta, DNA topoisomerase II beta, DNA topoisomerase II, 180 kD, DNA topoisomerase II, beta isozyme, EC 5.99.1.3, top2beta, TOPIIB, topo II beta, topoisomerase (DNA) II beta (180kD), topoisomerase (DNA) II beta 180kDa, topoisomerase II beta, topoisomerase IIb, U937 associated antigen

Gene Symbol

TOP2B

Additional TOP2B Products

Product Documents for TOP2B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TOP2B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TOP2B Antibody - BSA Free

Customer Reviews for TOP2B Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 08/04/2016
    TOP2B
    TOP2B Antibody - BSA Free NBP1-89527
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 06/29/2016
    TOP2B in Fibroblasts
    TOP2B Antibody - BSA Free NBP1-89527

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Protocols

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