TOPORS Antibody

Novus Biologicals | Catalog # NBP1-22976

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TOPORS Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 995 and 1045 of human topoisomerase I binding, arginine/serine-rich using the numbering given in entry topoisomerase I binding, arginine/serine-rich NP_005793.2 (GeneID 10210).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOPORS Antibody

Western Blot: TOPORS Antibody [NBP1-22976]

Western Blot: TOPORS Antibody [NBP1-22976]

Western Blot: TOPORS Antibody [NBP1-22976] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-TOPORS antibody NBP1-22976. TOPORS was also immunoprecipitated by another rabbit anti-TOPORS antibody.
Immunohistochemistry-Paraffin: TOPORS Antibody [NBP1-22976]

Immunohistochemistry-Paraffin: TOPORS Antibody [NBP1-22976]

Immunohistochemistry-Paraffin: TOPORS Antibody [NBP1-22976] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- TOPORS used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for TOPORS Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TOPORS

TOPORS (topoisomerase I-binding RS protein) was identified in a a yeast two-hybrid screen for proteins that bind the N-terminus of topoisomerase I. TOPORS contains a RING-type zinc-finger domain and an RS-rich region. TOPORS has been found to possess both ubiquitin and SUMO-1 E3 ligase activity. It can ubiquitinate and sumoylate p53 and may possibly function as a tumor suppressor. Defects in TOPORS are the cause of retinitis pigmentosa type 31.

Alternate Names

EC 6.3.2.-, LUNtopoisomerase I binding, arginine/serine-rich, p53-binding protein 3, P53BP3, retinitis pigmentosa 31 (autosomal dominant), RP31, SUMO1-protein E3 ligase Topors, topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase, Topoisomerase I-binding arginine/serine-rich protein, Topoisomerase I-binding RING finger protein, TP53BPLE3 ubiquitin-protein ligase Topors, Tumor suppressor p53-binding protein 3

Entrez Gene IDs

10210 (Human)

Gene Symbol

TOPORS

UniProt

Q9NS56 (Human)

Additional TOPORS Products

Product Documents for TOPORS Antibody

Certificate of Analysis

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Product Specific Notices for TOPORS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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