Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free
Novus Biologicals | Catalog # NBP2-61905
![Western Blot: Toxoplasma gondii ROP1 Antibody (4A7E12)BSA Free [NBP2-61905]](https://resources.rndsystems.com/images/products/Toxoplasma-gondii-ROP1-Antibody-4A7E12-Western-Blot-NBP2-61905-img0006.jpg "Western Blot: Toxoplasma gondii ROP1 Antibody (4A7E12)BSA Free [NBP2-61905]")
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Key Product Details
Species Reactivity
T. gondii
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 4A7E12
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of Toxoplasma gondii rhoptry protein Toxoplasma gondii ROP1 (AA: 42-183) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
42.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free
Western Blot: Toxoplasma gondii ROP1 Antibody (4A7E12)BSA Free [NBP2-61905]
Western Blot: Toxoplasma gondii ROP1 Antibody (4A7E12) [NBP2-61905] - Analysis using ROP1 mAb against HEK293 (1) and ROP1 (AA: 42-183)-hIgGFc transfected HEK293 (2) cell lysate.![Immunohistochemistry-Paraffin: Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free [NBP2-61905]](https://resources.rndsystems.com/images/products/Toxoplasma-gondii-ROP1-Antibody-4A7E12-Immunohistochemistry-NBP2-61905-img0004.jpg "Immunohistochemistry-Paraffin: Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free [NBP2-61905]")
Immunohistochemistry-Paraffin: Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free [NBP2-61905]
Immunohistochemistry-Paraffin: Toxoplasma gondii ROP1 Antibody (4A7E12) [NBP2-61905] - Analysis of esophageal cancer tissues using ROP1 mouse mAb with DAB staining.![Flow Cytometry: Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free [NBP2-61905]](https://resources.rndsystems.com/images/products/Toxoplasma-gondii-ROP1-Antibody-4A7E12-Flow-Cytometry-NBP2-61905-img0002.jpg "Flow Cytometry: Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free [NBP2-61905]")
Flow Cytometry: Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free [NBP2-61905]
Flow Cytometry: Toxoplasma gondii ROP1 Antibody (4A7E12) [NBP2-61905] - Analysis of MCF-7 cells using ROP1 mouse mAb (green) and negative control (red).![Western Blot: Toxoplasma gondii ROP1 Antibody (4A7E12)BSA Free [NBP2-61905]](https://resources.rndsystems.com/images/products/Toxoplasma-gondii-ROP1-Antibody-4A7E12-Western-Blot-NBP2-61905-img0005.jpg "Western Blot: Toxoplasma gondii ROP1 Antibody (4A7E12)BSA Free [NBP2-61905]")
Western Blot: Toxoplasma gondii ROP1 Antibody (4A7E12)BSA Free [NBP2-61905]
Western Blot: Toxoplasma gondii ROP1 Antibody (4A7E12) [NBP2-61905] - Analysis using ROP1 mAb against Toxoplasma gondii rhoptry protein ROP1 (AA: 42-183) recombinant protein. (Expected MW is 41.1 kDa)![Western Blot: Toxoplasma gondii ROP1 Antibody (4A7E12)BSA Free [NBP2-61905]](https://resources.rndsystems.com/images/products/Toxoplasma-gondii-ROP1-Antibody-4A7E12-Western-Blot-NBP2-61905-img0007.jpg "Western Blot: Toxoplasma gondii ROP1 Antibody (4A7E12)BSA Free [NBP2-61905]")
Western Blot: Toxoplasma gondii ROP1 Antibody (4A7E12)BSA Free [NBP2-61905]
Western Blot: Toxoplasma gondii ROP1 Antibody (4A7E12) [NBP2-61905] - Analysis using ROP1 mouse mAb against BCBL-1 (1) and Raw264.7 (2) cell lysate.![Immunohistochemistry-Paraffin: Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free [NBP2-61905]](https://resources.rndsystems.com/images/products/Toxoplasma-gondii-ROP1-Antibody-4A7E12-Immunohistochemistry-NBP2-61905-img0003.jpg "Immunohistochemistry-Paraffin: Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free [NBP2-61905]")
Immunohistochemistry-Paraffin: Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free [NBP2-61905]
Immunohistochemistry-Paraffin: Toxoplasma gondii ROP1 Antibody (4A7E12) [NBP2-61905] - Analysis of breast cancer tissues using ROP1 mouse mAb with DAB staining.![ELISA: Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free [NBP2-61905]](https://resources.rndsystems.com/images/products/Toxoplasma-gondii-ROP1-Antibody-4A7E12-ELISA-NBP2-61905-img0001.jpg "ELISA: Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free [NBP2-61905]")
ELISA: Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free [NBP2-61905]
ELISA: Toxoplasma gondii ROP1 Antibody (4A7E12) [NBP2-61905] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)Applications for Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:100-1:2000
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Toxoplasma gondii ROP1
Alternate Names
Toxoplasma gondii rhoptry protein
Gene Symbol
ROP1
Additional Toxoplasma gondii ROP1 Products
Product Documents for Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Toxoplasma gondii ROP1 Antibody (4A7E12) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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