TPD52L1/D53 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84313

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TPD52L1/D53 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MEAQAQGLLETEPLQGTDEDAVASADFSSMLSEEEKEELKAELVQLEDEITTLRQVLSAKERHLVEI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TPD52L1/D53 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TPD52L1/D53 Antibody [NBP1-84313]

Immunocytochemistry/ Immunofluorescence: TPD52L1/D53 Antibody [NBP1-84313]

Immunocytochemistry/Immunofluorescence: TPD52L1/D53 Antibody [NBP1-84313] - Staining of human cell line U-251 MG shows localization to plasma membrane, cytosol and cell junctions.
Immunohistochemistry-Paraffin: TPD52L1/D53 Antibody [NBP1-84313]

Immunohistochemistry-Paraffin: TPD52L1/D53 Antibody [NBP1-84313]

Immunohistochemistry-Paraffin: TPD52L1/D53 Antibody [NBP1-84313] - Staining of human lymph node shows low expression as expected.
Immunohistochemistry-Paraffin: TPD52L1/D53 Antibody [NBP1-84313]

Immunohistochemistry-Paraffin: TPD52L1/D53 Antibody [NBP1-84313]

Immunohistochemistry-Paraffin: TPD52L1/D53 Antibody [NBP1-84313] - Staining of human salivary gland shows high expression.
TPD52L1/D53 Antibody - BSA Free Immunohistochemistry-Paraffin: TPD52L1/D53 Antibody - BSA Free [NBP1-84313]

Immunohistochemistry-Paraffin: TPD52L1/D53 Antibody - BSA Free [NBP1-84313]

Analysis in human salivary gland and lymph node tissues using Anti-TPD52L1 antibody. Corresponding TPD52L1 RNA-seq data are presented for the same tissues.
TPD52L1/D53 Antibody - BSA Free Western Blot: TPD52L1/D53 Antibody - BSA Free [NBP1-84313]

Western Blot: TPD52L1/D53 Antibody - BSA Free [NBP1-84313]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line MCF-7

Applications for TPD52L1/D53 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TPD52L1/D53

TPD52L1/D53 encodes a member of the tumor protein D52 (TPD52) family. The encoded protein contains a coiled-coil domain and may form homo- or hetero-dimer with TPD52 family members. The protein is reported to be involved in cell proliferation and calcium signaling. It also interacts with the mitogen-activated protein kinase kinase kinase 5 (MAP3K5/ASK1) and positively regulates MAP3K5-induced apoptosis. Multiple alternatively spliced transcript variants have been observed, but the full-length nature of some variants has not yet been determined. [provided by RefSeq]

Alternate Names

D53, MGC8556, tumor protein D52-like 1hD53TPD52L2, tumor protein D52-like 2, tumor protein D53

Gene Symbol

TPD52L1

Additional TPD52L1/D53 Products

Product Documents for TPD52L1/D53 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TPD52L1/D53 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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