TPMT Antibody (1D4) - Azide and BSA Free

Novus Biologicals | Catalog # H00007172-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D4

Format

Azide and BSA Free
View all TPMT Primary Antibodies »

Product Specifications

Immunogen

TPMT (AAH05339, 1 a.a. ~ 245 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQGHQLLKKHLDTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTEQNLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRGALVAINPGDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGKICNIRRLEKVDAFEERHKSWGIDCLFEKLYLLTEK

Specificity

TPMT - thiopurine S-methyltransferase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TPMT Antibody (1D4) - Azide and BSA Free

Western Blot: TPMT Antibody (1D4) [H00007172-M02]

Western Blot: TPMT Antibody (1D4) [H00007172-M02]

Western Blot: TPMT Antibody (1D4) [H00007172-M02] - Analysis of TPMT expression in transfected 293T cell line by TPMT monoclonal antibody (M02), clone 1D4. Lane 1: TPMT transfected lysate(28.2 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: TPMT Antibody (1D4) [H00007172-M02]

Immunocytochemistry/ Immunofluorescence: TPMT Antibody (1D4) [H00007172-M02]

Immunocytochemistry/Immunofluorescence: TPMT Antibody (1D4) [H00007172-M02] - Analysis of monoclonal antibody to TPMT on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: TPMT Antibody (1D4) [H00007172-M02] - Detection limit for recombinant GST tagged TPMT is approximately 1ng/ml as a capture antibody.

Applications for TPMT Antibody (1D4) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TPMT

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.

Long Name

Thiopurine S-methyltransferase

Alternate Names

EC 2.1.1.67

Entrez Gene IDs

7172 (Human)

Gene Symbol

TPMT

OMIM

187680 (Human)

UniProt

AAH05339 (Human)

Additional TPMT Products

Product Documents for TPMT Antibody (1D4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TPMT Antibody (1D4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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