TPT1/TCTP Antibody (10A9) - BSA Free

Novus Biologicals | Catalog # NBP3-26272

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10A9 expressed in HEK293

Format

BSA Free
View all TPT1/TCTP Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human TPT1/TCTP [UniProt P13693]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TPT1/TCTP Antibody (10A9) - BSA Free

TPT1/TCTP Antibody (10A9)

Immunoprecipitation: TPT1/TCTP Antibody (10A9) [NBP3-26272] -

Immunoprecipitation: TPT1/TCTP Antibody (10A9) [NBP3-26272] - Immunoprecipitating TPT1/TCTP in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26272 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26272 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).
TPT1/TCTP Antibody (10A9)

Immunohistochemistry: TPT1/TCTP Antibody (10A9) [NBP3-26272] -

Immunohistochemistry: TPT1/TCTP Antibody (10A9) [NBP3-26272] - Image of TPT1/TCTP Antibody (10A9) diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
TPT1/TCTP Antibody (10A9)

Western Blot: TPT1/TCTP Antibody (10A9) [NBP3-26272] -

Western Blot: TPT1/TCTP Antibody (10A9) [NBP3-26272] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue.
All lanes: TPT1/TCTP Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 20, 16 kDa
Observed band size: 25 kDa
TPT1/TCTP Antibody (10A9)

Immunocytochemistry/Immunofluorescence: TPT1/TCTP Antibody (10A9) [NBP3-26272] -

Immunocytochemistry/Immunofluorescence: TPT1/TCTP Antibody (10A9) [NBP3-26272] - Staining of SY5Y Cells with TPT1/TCTP Antibody (10A9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for TPT1/TCTP Antibody (10A9) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: TPT1/TCTP

Histamine is an inflammatory mediator that is ubiquitously expressed and has a broad range of pharmacologic effects. Specifically, it plays a role in the central nervous, gastrointestinal, respiratory and immune systems. Histamine release is mediated by the stimulation of mast cells and basophils. Histamine-releasing factor (HRF) is a cytokine-like molecule that causes the release of histamine, IL-4 and IL-13 from basophils as well as the secretion of IL-8 and a calcium response in eosinophils. HRF belongs to the translationally controlled tumor protein (TCTP) family and is detected as a protein of 21 kDa in mice and 23 kDa in human. It is expressed in several healthy and tumoral cells, including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas and lymphomas, and it is localized in the cytoplasm. HRF plays a pivotal role in allergic diseases, and due to its wide distribution in brain, is thought to be involved in neurodegenerative disorders, such as Alzheimer's disease and Down Syndrome.

Long Name

Translationally-controlled Tumor Protein 1

Alternate Names

Fortilin, HRF, p02, TCTP

Gene Symbol

TPT1

Additional TPT1/TCTP Products

Product Documents for TPT1/TCTP Antibody (10A9) - BSA Free

Certificate of Analysis

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Product Specific Notices for TPT1/TCTP Antibody (10A9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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