TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) - BSA Free
Novus Biologicals | Catalog # NBP2-79822
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2A Clone # H43
Format
BSA Free
Loading...
Product Specifications
Immunogen
Synthetic peptide from the full length Human Thyroid hormone receptor protein
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images
Western Blot: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822]
Western Blot: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Western Blot analysis of Human Hep G2 Hepatoblastoma Cell lysate showing detection of TR alpha/NR1A1/Thyroid Hormone Receptor alpha protein using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Load: 10 ug. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Other Band(s): Higher molceular weight bands could be due to PTMs.Immunocytochemistry/ Immunofluorescence: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822]
Immunocytochemistry/Immunofluorescence: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody. (D) Hoechst (E) Composite.Immunohistochemistry: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822]
Immunohistochemistry: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Immunohistochemistry analysis using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 4X.Immunohistochemistry: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822]
Immunohistochemistry: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Immunohistochemistry analysis using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 7.5X.Applications
Application
Recommended Usage
Immunohistochemistry
1:100
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 °C.
Background: TR alpha/NR1A1
Long Name
Thyroid Hormone Receptor alpha
Alternate Names
NR1A1, THRA, v-ErbA1
Gene Symbol
THRA
Additional TR alpha/NR1A1 Products
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews
There are currently no reviews for this product. Be the first to review TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) - BSA Free and earn rewards!
Have you used TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...