TR4/NR2C2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81658

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TR4/NR2C2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DGARQTGLLDPGMLVNIQQPLIREDGTVLLATDSKAETSQGALGTLANVVTSLANLSESLNNGDTSEIQPEDQSASEITRAFDTLAKALNTTDSSSSPSLADGIDTSGGGSIHVISRDQSTPIIEVEGPLLSDTHVTFKLTMPSPMP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TR4/NR2C2 Antibody - BSA Free

Western Blot: TR4/NR2C2 Antibody [NBP1-81658]

Western Blot: TR4/NR2C2 Antibody [NBP1-81658]

Western Blot: TR4/NR2C2 Antibody [NBP1-81658] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: TR4/NR2C2 Antibody [NBP1-81658]

Immunocytochemistry/ Immunofluorescence: TR4/NR2C2 Antibody [NBP1-81658]

Immunocytochemistry/Immunofluorescence: TR4/NR2C2 Antibody [NBP1-81658] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Western Blot: TR4/NR2C2 Antibody [NBP1-81658]

Western Blot: TR4/NR2C2 Antibody [NBP1-81658]

Western Blot: TR4/NR2C2 Antibody [NBP1-81658] - Analysis in human cell line HeLa.
TR4/NR2C2 Antibody

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP1-81658] -

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP1-81658] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
TR4/NR2C2 Antibody

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP1-81658] -

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP1-81658] -Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.
TR4/NR2C2 Antibody

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP1-81658] -

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP1-81658] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
TR4/NR2C2 Antibody

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP1-81658] -

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP1-81658] - Staining of human rectum shows strong nuclear positivity in glandular cells.
TR4/NR2C2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: TR4/NR2C2 Antibody - BSA Free [NBP1-81658]

Chromatin Immunoprecipitation-exo-Seq: TR4/NR2C2 Antibody - BSA Free [NBP1-81658]

ChIP-Exo-Seq composite graph for Anti-NR2C2 (NBP1-81658) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for TR4/NR2C2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TR4/NR2C2

TR4 (TAK1, NR2C2) is an orphan nuclear receptor. TR4 was originally cloned from lymphoblastoma Raji cells or mouse brain cDNA library. No ligand has been reported. Northern blot shows TR4 is transcribed as a 9kb mRNA in many tissues and as a 2.8kb mRNA in testis, mainly in spermatocytes. TR4 has two isoforms called TR4alpha1 and TR4 alpha2,which differ in 19 amino acids coded by two separate exons. Both products translated from the 9kb transcript are ubiquitously expressed. Since TR4 binds to the same elements for the RAR-RXR or TR-RXR heterodimers, TR4 may have an inhibitory effect for retinoic-acid mediated transactivation.

Long Name

Testicular Orphan Nuclear Receptor 4

Alternate Names

NR2C2, TAK1

Gene Symbol

NR2C2

Additional TR4/NR2C2 Products

Product Documents for TR4/NR2C2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TR4/NR2C2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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