TRA2A Antibody - Azide and BSA Free

Western Blot: TRA2A Antibody [H00029896-B01P]

Western Blot: TRA2A Antibody [H00029896-B01P] - Analysis of TRA2A expression in HepG2.

Western Blot: TRA2A Antibody [H00029896-B01P]

Western Blot: TRA2A Antibody [H00029896-B01P] - Analysis of TRA2A expression in Raw 264.7.

Western Blot: TRA2A Antibody [H00029896-B01P]

Western Blot: TRA2A Antibody [H00029896-B01P] - Analysis of TRA2A expression in transfected 293T cell line by TRA2A polyclonal antibody. Lane 1: TRA2A transfected lysate(31.13 KDa). Lane 2: Non-transfected lysate.

Knockdown Validated: TRA2A Antibody - Azide and BSA Free [H00029896-B01P] -

Tra2 beta regulates Tra2 alpha protein expression.(a) Western blot analysis showing depletion of TRA2B induces reciprocal upregulation of Tra2 alpha protein expression, whereas depletion of TRA2A had minimal effect on Tra2 beta protein expression. (b) Quantitation of cross-regulation between Tra2 alpha and Tra2 beta at the protein level (Tra2 alpha and Tra2 beta protein expression were quantified relative to alpha –Tubulin from three western blots using independent biological replicates). (c) Quantitation of cross-regulation between Tra2 alpha and Tra2 beta at the RNA level from quantitative PCR analysis of three independent biological replicates in MDA-MB-231 cells. (d) Splicing inclusion of the TRA2A poison exon is strongly reduced by depletion of endogenous Tra2 beta protein, whereas splicing inclusion of the TRA2B poison exon is less affected by depletion of Tra2 alpha protein. Splicing patterns were monitored by RT–PCR between flanking exons (arrowed) followed by capillary electrophoresis. (e) Screenshot from the UCSC genome browser35 showing the TRA2B and TRA2A genes, and the positions of aligned RNA-seq reads (green peaks) and Tra2 beta binding (orange clusters of significant cross-linking by Tra2 beta protein identified by biological triplicate iCLIP experiments) in MDA-MB-231 cells. Probability (P) values were calculated using an independent two-sample t-test between negative control siRNA-treated cells and the gene-specific siRNA-treated cells (statistical significance shown as: *P<0.05, **P<0.01, ***P<0.0001). All data represented by bar charts was generated from three biological replicates and error bars represent the s.e.m. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25208576), licensed under a CC-BY license. Not internally tested by Novus Biologicals.