TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76985

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Flow Cytometry, Flow (Cell Surface)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRAILR4/TNFRSF10D Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid peptide near the center of human DcR2. The immunogen is located within amino acids 230 - 280 of DcR2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free

Western Blot: TRAILR4/TNFRSF10D/DcR2 AntibodyBSA Free [NBP1-76985]

Western Blot: TRAILR4/TNFRSF10D/DcR2 AntibodyBSA Free [NBP1-76985]

Western Blot: TRAILR4/TNFRSF10D/DcR2 Antibody [NBP1-76985] - Analysis of 50 ug of whole cell lysate from HeLa cells with anti-DcR2 at 1:1000 dilution.
Immunocytochemistry/ Immunofluorescence: TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free [NBP1-76985]

Immunocytochemistry/ Immunofluorescence: TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free [NBP1-76985]

Immunocytochemistry/Immunofluorescence: TRAILR4/TNFRSF10D/DcR2 Antibody [NBP1-76985] - Staining of HeLa cells using DcR2 antibody at 10ug/ml.
TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free

Immunohistochemistry: TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free [NBP1-76985] -

Immunohistochemistry: TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free [NBP1-76985] - Immunohistochemistry of TRAILR4/TNFRSF10D/DcR2 in mouse kidney node tissue with TRAILR4/TNFRSF10D/DcR2 antibody at 2.5 ug/ml.
TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free [NBP1-76985] -

Immunocytochemistry/ Immunofluorescence: TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free [NBP1-76985] - Immunofluorescence of TRAILR4/TNFRSF10D/DcR2 in mouse kidney tissue withTRAILR4/TNFRSF10D/DcR2 antibody at 20 ug/ml.

Green: TRAILR4/TNFRSF10D/DcR2 Antibody
Blue: DAPI staining
TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free

Western Blot: TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free [NBP1-76985] -

Effects of 2ME and DMOG administration on TRAIL pathway and neuronal apoptosis 72 h after TBI. (A) Representative Western blot images. (B) Densitometric quantification of HIF-1 alpha. (C) Densitometric quantification of DcR1. (D) Densitometric quantification of DR5. (E) Densitometric quantification of TRAIL. (F) Densitometric quantification of DcR2. (G) Densitometric quantification of cleaved caspase-3. (H) Quantification of DcR1-positive cells. (I) Representative microphotographs of immunofluorescence staining showing localization of DcR1 (red) and NeuN (green) in injured cerebral cortex after TBI. Scale bar = 100 μm. Arrow indicates the DcR1 positive cell. *p < 0.05 vs. sham; #p < 0.05 vs. TBI + Vehicle; &p < 0.05 vs. TBI + 2ME; ns, no significance vs. sham. N = 6 per group. Data are represented as mean +/- SD. *p < 0.05 vs. TBI + Vehicle. One-way ANOVA, Tukey’s post hoctest. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32848609), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1 ug/ml
Application Notes
Use in FLOW reported in scientific literature (PMID: 12795746). Use in Flow-cell surface reported in scientific literature (PMID: 12173037).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRAILR4/TNFRSF10D

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL, and DcR1/TRID is a decoy receptor. Another member of the TRAIL receptor family was more recently identified and designated DcR2, TRAIL-R4, or TRUNDD. DcR2 has an extracellular TRAIL-binding domain but lacks intracellular death domain and does not induce apoptosis. Like DR4 and DR5, DcR2 transcript is widely expressed in normal human tissues. Overexpression of DcR2 attenuated TRAIL-induced apoptosis.

Long Name

TRAIL Receptor 4

Alternate Names

CD264, DcR2, TNFRSF10D, TRAIL R4

Gene Symbol

TNFRSF10D

UniProt

Q9UBN6

Additional TRAILR4/TNFRSF10D Products

Product Documents for TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free

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Protocols

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