DuoSet IC (Intracellular) Active Transcription Factor Assays use a capture antibody, a biotinylated DNA oligonucleotide, and streptavidin HRP to demonstrate relative transcription factor activity. These Active Transcription Factor Kits offer a reliable alternative to electrophoretic mobility shift assays (EMSA). Several DuoSet IC transcription factor assays can also be easily converted to measure total (active and inactive) transcription factor (see product data sheets for details).
Optimized antibody pairings and recommended concentrations save lengthy development time
Mass-calibrated standards allow for consistent results and eliminate vial-to-vial variation
Generic development protocols provide direction to start an optimization protocol
Customize the assay to your specific needs
Available in 2-, 5-, and 15-(96-well) plate pack sizes
Excellent alternative to EMSA
Biotin Labeled double stranded oligonucleotide
Unlabeled double stranded oligonucleotide
*Kit contents may vary slightly. Please refer to product-specific inserts for a complete list of contents for individual assays.