Transcription Factor Binding Assays

Assay Principle

View Assay Principle

DuoSet IC (Intracellular) Active Transcription Factor Assays use a capture antibody, a biotinylated DNA oligonucleotide, and streptavidin HRP to demonstrate relative transcription factor activity. These Active Transcription Factor Kits offer a reliable alternative to electrophoretic mobility shift assays (EMSA). Several DuoSet IC transcription factor assays can also be easily converted to measure total (active and inactive) transcription factor (see product data sheets for details).


  • Optimized antibody pairings and recommended concentrations save lengthy development time
  • Mass-calibrated standards allow for consistent results and eliminate vial-to-vial variation
  • Generic development protocols provide direction to start an optimization protocol
  • Customize the assay to your specific needs
  • Available in 2-, 5-, and 15-(96-well) plate pack sizes
  • Excellent alternative to EMSA

Kit Contents*

  • Capture Antibody
  • Biotin Labeled double stranded oligonucleotide
  • Unlabeled double stranded oligonucleotide
  • Streptavidin-HRP

*Kit contents may vary slightly. Please refer to product-specific inserts for a complete list of contents for individual assays.