Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25726

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (97%), Porcine (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1270 expressed in HEK293

Format

Azide and BSA Free
View all Transgelin/TAGLN Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transgelin/TAGLN/SM22 alpha. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%), Xenopus (80%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free

Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free [NBP3-25726] -

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free [NBP3-25726] - Transgelin antibody [HL1270] detects Transgelin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse smooth muscle. Transgelin stained by Transgelin antibody [HL1270] (NBP3-25726) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free [NBP3-25726] -

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free [NBP3-25726] - Transgelin antibody [HL1270] detects Transgelin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. Transgelin stained by Transgelin antibody [HL1270] (NBP3-25726) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free [NBP3-25726] -

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free [NBP3-25726] - Transgelin antibody [HL1270] detects Transgelin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Transgelin stained by Transgelin antibody [HL1270] (NBP3-25726) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free [NBP3-25726] -

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free [NBP3-25726] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody [HL1270] (NBP3-25726) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free [NBP3-25726] -

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free [NBP3-25726] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody [HL1270] (NBP3-25726) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free [NBP3-25726] -

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free [NBP3-25726] - Transgelin antibody [HL1270] detects Transgelin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse smooth muscle. Transgelin stained by Transgelin antibody [HL1270] (NBP3-25726) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Transgelin/TAGLN

In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22 kDa protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus.The protein is a transformation and shape-change sensitive actin cross-linking/geling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

Alternate Names

SM22 alpha, TAGLN, WS3-10

Gene Symbol

TAGLN

Additional Transgelin/TAGLN Products

Product Documents for Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Transgelin/TAGLN/SM22 alpha Antibody (HL1270) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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