Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-20101

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Mass Spectrometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SAIC-33B-129

Format

Azide and BSA Free
View all Transgelin/TAGLN Primary Antibodies »

Product Specifications

Immunogen

Peptide "AAEDYGVIK", derived from the actin cross-linking protein Transgelin, conjugated to KLH.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images

Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] -

Immunocytochemistry/Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-33B-129 (NBP3-20101) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20101, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] -

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] - HeLa(A), Caco-2(B), U2OS(C) cell lysate and human placenta(D), muscle(E) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-33B-129 (NBP3-20101) at 0.0003 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free

Flow Cytometry: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] -

Flow Cytometry: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-33B-129 (NBP3-20101, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Mass Spectrometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Ab can be used in immunoMRM.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Transgelin/TAGLN

In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22 kDa protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus.The protein is a transformation and shape-change sensitive actin cross-linking/geling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

Alternate Names

SM22 alpha, TAGLN, WS3-10

Gene Symbol

TAGLN

Additional Transgelin/TAGLN Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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