Transglutaminase 5 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-94524
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 461-720 of human TGM5 (NP_963925.2). LQKLKARSFHGSQRGAELQPSRPTSLSQDSPRSLHTPSLRPSDVVQVSLKFKLLDPPNMGQDICFVLLALNMSSQFKDLKVNLSAQSLLHDGSPLSPFWQDTAFITLSPKEAKTYPCKISYSQYSQYLSTDKLIRISALGEEKSSPEKILVNKIITLSYPSITINVLGAAVVNQPLSIQVIFSNPLSEQVEDCVLTVEGSGLFKKQQKVFLGVLKPQHQASIILETVPFKSGQRQIQANMRSNKFKDIKGYRNVYVDFAL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Transglutaminase 5 Antibody - BSA Free
Western Blot: Transglutaminase 5 Antibody - BSA Free [Transglutaminase 5] -
Western Blot: Transglutaminase 5 Antibody - BSA Free [Transglutaminase 5] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Transglutaminase 5 using Transglutaminase 5 Rabbit pAb at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
![Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody - BSA Free [NBP2-94524]](https://resources.rndsystems.com/images/products/Transglutaminase-5-Antibody-Immunohistochemistry-Paraffin-NBP2-94524-img0003.jpg "Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody - BSA Free [NBP2-94524]")
Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody - BSA Free [NBP2-94524]
Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded rat kidney using Transglutaminase 5.![Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody - BSA Free [NBP2-94524]](https://resources.rndsystems.com/images/products/Transglutaminase-5-Antibody-Immunohistochemistry-Paraffin-NBP2-94524-img0001.jpg "Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody - BSA Free [NBP2-94524]")
Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody - BSA Free [NBP2-94524]
Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded human colon using Transglutaminase 5.![Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody - BSA Free [NBP2-94524]](https://resources.rndsystems.com/images/products/Transglutaminase-5-Antibody-Immunohistochemistry-Paraffin-NBP2-94524-img0002.jpg "Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody - BSA Free [NBP2-94524]")
Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody - BSA Free [NBP2-94524]
Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded mouse heart using Transglutaminase 5.Applications for Transglutaminase 5 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:2000 - 1:8000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Transglutaminase 5/TGM5
Long Name
Transglutaminase 5
Alternate Names
PSS2, TGASE5, TGASEX, TGM5
Gene Symbol
TGM5
Additional Transglutaminase 5/TGM5 Products
Product Documents for Transglutaminase 5 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Transglutaminase 5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Transglutaminase 5 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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