TRAP220/MED1 Antibody (BLR037F)

Novus Biologicals | Catalog # NBP2-76407

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR037F
View all TRAP220 Primary Antibodies »

Product Specifications

Immunogen

Immunogen sequence between amino acids 1550 amd 1581

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAP220/MED1 Antibody (BLR037F)

Western Blot: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407]

Western Blot: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407]

Western Blot: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407] - Detection of human MED1 by WB of HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat lysate. Antibody: Rabbit anti-MED1 recombinant monoclonal [BLR037F] (NBP2-76407). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407]

Immunocytochemistry: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407]

Immunocytochemistry: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407] - Detection of human MED1 in FFPE HCT 116 cells by ICC. Antibody: Rabbit anti-MED1 recombinant monoclonal [BLR037F] (NBP2-76407). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407]

Immunohistochemistry: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407]

Immunohistochemistry: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407] - Detection of mouse MED1 in FFPE mouse renal cell carcinoma by IHC. Antibody: Rabbit anti-MED1 recombinant monoclonal [BLR037F] (NBP2-76407). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407]

Immunohistochemistry: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407]

Immunohistochemistry: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407] - Detection of human MED1 in FFPE small cell lung cancer by IHC. Antibody: Rabbit anti-MED1 recombinant monoclonal [BLR037F] (NBP2-76407). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunoprecipitation: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407]

Immunoprecipitation: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407]

Immunoprecipitation: TRAP220/MED1 Antibody (BLR037F) [NBP2-76407] - Detection of human MED1 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-MED1 recombinant monoclonal [BLR037F] (NBP2-76407) and rabbit anti-MED1 (NB100-2574).

Applications for TRAP220/MED1 Antibody (BLR037F)

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunoprecipitation

20 ul/1 mg lysate

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: TRAP220

MED1 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Long Name

Thyroid Hormone Receptor-associated Protein Complex Component 220 kDa

Alternate Names

DRIP230, MBD4, MED1, PPARBP, PPARGBP, TRIP2

Gene Symbol

MED1

Additional TRAP220 Products

Product Documents for TRAP220/MED1 Antibody (BLR037F)

Certificate of Analysis

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Product Specific Notices for TRAP220/MED1 Antibody (BLR037F)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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