Trappin-2/Elafin/Skalp Antibody (TRAB2F)

Novus Biologicals | Catalog # NBP3-11231

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunoassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # TRAB2F
View all Trappin-2/Elafin Primary Antibodies »

Product Specifications

Immunogen

TRAB2F is an Elafin/SKALP specific antibody which shows strong reaction with fully processed Elafin/SKALP.

Specificity

TRAB2F shows strong reaction with fully processed Elafin/SKALP (C-terminal 57 aa) and a weaker reaction with human native full-length Elafin/SKALP.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Trappin-2/Elafin/Skalp Antibody (TRAB2F)

Application
Recommended Usage

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Immunohistochemistry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For Western blotting it is recommended to use non-reducing conditions.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Trappin-2/Elafin

Elafin is an epithelial proteinase inhibitor also known under various other names such as Skin-derived Anti leukoproteinase (SKALP) and Elastase-Specific Inhibitor (ESI). Elafin belongs to the Trappin gene family and was given the systematic name Trappin-2. The Trappin family is defined by a N-terminal transglutaminase substrate domain and a C-terminal four disulphide core. Trappins have been suggested to play a role in the regulation of inflammation and in protection against tissue damage in stratified epithelia. Elafin is an inhibitor of leukocyte elastase and proteinase-3 and is a substrate for transglutaminases. The protein is constitutively expressed in various epithelia including hair follicles, oesophagus, vagina and oral cavity. Elafin is not present in normal human skin but is strongly induced during inflammation as in psoriasis and wound healing. Antibodies to Elafin can be used to evaluate the effects of treatment of psoriasis since its expression is significantly correlated with clinical scores. Antibodies to Elafin have also been succesfully used to study differentiation in squamous cell carcinoma of the head-and-neck region, oesophagus and skin. It is also shown that Elafin possesses antimicrobial activity against gram-positive and gram-negative bacteria. TRAB2F is an Elafin/SKALP specific antibody which shows strong reaction with fully processed Elafin/SKALP (C-terminal 57 aa) and a weaker reaction with human native full-length Elafin/SKALP. The antibody can be used for purification of human native full-length Elafin/SKALP. For detection of human native full-length Elafin/SKALP we advise to use TRAB2O.

Long Name

Elastase Inhibitor, Transglutaminase Substrate, Serine Protease

Alternate Names

Elafin, ESI, PI3, SKALP, Trappin2

Gene Symbol

PI3

Additional Trappin-2/Elafin Products

Product Documents for Trappin-2/Elafin/Skalp Antibody (TRAB2F)

Certificate of Analysis

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Product Specific Notices for Trappin-2/Elafin/Skalp Antibody (TRAB2F)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Serine Proteases and Regulators

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Protocols

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FAQs

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