TRERF1 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-81655
![Western Blot: TRERF1 Antibody [NB100-81655]](https://resources.rndsystems.com/images/products/TRERF1-Antibody-Western-Blot-NB100-81655-img0001.jpg "Western Blot: TRERF1 Antibody [NB100-81655]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1150 and 1200 of human transcriptional regulating protein 132 (transcriptional regulating factor 1) using the numbering given in entry NP_277037.1 (GeneID 55809).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TRERF1 Antibody - BSA Free
![Immunohistochemistry: TRERF1 Antibody [NB100-81655]](https://resources.rndsystems.com/images/products/TRERF1-Antibody-Immunohistochemistry-NB100-81655-img0004.jpg "Immunohistochemistry: TRERF1 Antibody [NB100-81655]")
Immunohistochemistry: TRERF1 Antibody [NB100-81655]
Immunohistochemistry: TRERF1 Antibody [NB100-81655] - Detection of human TReP-132/TRERF1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-TReP-132/TRERF1 (NB100-81655). Detection: DAB![Immunohistochemistry: TRERF1 Antibody [NB100-81655]](https://resources.rndsystems.com/images/products/TRERF1-Antibody-Immunohistochemistry-NB100-81655-img0003.jpg "Immunohistochemistry: TRERF1 Antibody [NB100-81655]")
Immunohistochemistry: TRERF1 Antibody [NB100-81655]
Immunohistochemistry: TRERF1 Antibody [NB100-81655] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- TReP-132/TRERF1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DABApplications for TRERF1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:2000 to 1:10000
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
NB100-81655 can be used in (Co-IP application PMID: 21258344).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TRERF1
Alternate Names
BCAR2, Breast cancer estrogen resistance 2RAPAHSA277276, dJ139D8.5, FLJ21198, rapa-1, rapa-2, RP1-139D8.5, transcriptional regulating factor 1, transcriptional regulating protein 132, transcriptional-regulating factor 1, Transcriptional-regulating protein 132, TREP132, TReP-132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132
Entrez Gene IDs
55809 (Human)
Gene Symbol
TRERF1
UniProt
Additional TRERF1 Products
Product Documents for TRERF1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TRERF1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for TRERF1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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