TREX1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76977

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TREX1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide near the center of human TREX1. The immunogen is located within amino acids 220 - 270 of TREX1. Amino Acid Squence: TRLYGQSPPDSHT

Specificity

TREX1 antibody will not cross-react with the related protein TREX2. At least three isoforms of TREX1 are known to exist; this antibody will recognize all three isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TREX1 Antibody - BSA Free

Western Blot: TREX1 AntibodyBSA Free [NBP1-76977]

Western Blot: TREX1 AntibodyBSA Free [NBP1-76977]

Western Blot: TREX1 Antibody [NBP1-76977] - Analysis of human spleen tissue lysate with TREX1 antibody at (A) 0.5 ug/mL and (B) 1 ug/mL.
TREX1 Antibody - BSA Free

Immunohistochemistry: TREX1 Antibody - BSA Free [NBP1-76977] -

Immunohistochemistry: TREX1 Antibody - BSA Free [NBP1-76977] - Immunohistochemistry of TREX1 in human spleen tissue with TREX1 antibody at 2.5 u/mL.
TREX1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TREX1 Antibody - BSA Free [NBP1-76977] -

Immunocytochemistry/ Immunofluorescence: TREX1 Antibody - BSA Free [NBP1-76977] - Immunofluorescence of TREX1 in Human Spleen tissue with TREX1 antibody at 20 ug/mL.
TREX1 Antibody - BSA Free

Western Blot: TREX1 Antibody - BSA Free [NBP1-76977] -

DNA damage plays an important role in radiation-induced immune activation. A Schematic representation of irradiated tumor cells inducing maturation of murine bone marrow-derived DCs. Flow peak of a proportion of CD86+ CD80+ DC in each group. The proportion of CD86+ CD80+ DC in each group. Flow cytometric analysis of CD86+ CD80+ DC in each group. n = 6. B Levels of dsDNA in culture supernatant and cytoplasm of tumor cells in each group. C Expression and quantification of DNA damage and repair proteins. D Expression and quantification of cGAS-STING pathway proteins. E Expression and quantification of DAMPs. F Relative expression levels of HSP70 and HSP90 in the supernatants after different treatments. ns indicates no statistical difference. *, P < 0.05. **, P < 0.01. ***, P < 0.001. The statistical test was performed using One-way ANOVA, followed by Tukey’s multiple comparisons test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39736508), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TREX1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/mL

Western Blot

0.5 - 1 ug/mL

Reviewed Applications

Read 1 review rated 4 using NBP1-76977 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TREX1

Trex1 is the major human 3' to 5' exonuclease which is required for checkpoint signaling after DNA damage. It is ubiquitously expressed, binds to single stranded DNA coated with replication protein A that accumulates at sites of DNA damage and recruits the ataxia telangiectasia and Rad3 related protein (ATR), a checkpoint kinase, to sites of DNA damage and replication stress. Trex1 is required for ATR expression. This gene uses two different open reading frames. The upstream ORF encodes proteins which interact with ATR and localize to intranuclear foci induced by DNA damage and are essential components of the DNA damage checkpoint. The downstream ORF encodes proteins with 3' to 5' exonuclease activity and may be a subunit of human DNA polymerase III. Multiple transcript variants encoding different isoforms have been found. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis. TREX1 antibody will not cross-react with the related protein TREX2.

Alternate Names

3'-5' exonuclease TREX1, AGS1, Aicardi-Goutieres syndrome 1, CRV, DKFZp434J0310, DNase III, DRN3, EC 3.1.11.2, HERNS, three prime repair exonuclease 1,3' repair exonuclease 1

Gene Symbol

TREX1

UniProt

NP_057465 (Human)

Additional TREX1 Products

Product Documents for TREX1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TREX1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TREX1 Antibody - BSA Free

Customer Reviews for TREX1 Antibody - BSA Free (1)

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  • TREX1 Antibody
    Name: Prashant Rai
    Application: Western Blot
    Sample Tested: MEFs whole cell lysate
    Species: Mouse
    Verified Customer | Posted 07/17/2019
    Lane 1 and 2: MEF line#1 Lane 3 and 4: MEF line#2 Beta-actin is used as loading control
    Cell lysates run on Bis-Tris 4-12% gradient gel
    TREX1 Antibody - BSA Free NBP1-76977

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Protocols

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