Triadin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87417

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Triadin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VSTRKESLQLHNVTKAEKPARVSKDVEDVPASKKAKEGTEDVSPTKQKSPISFFQCVYLDGYNGYGFQFP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Triadin Antibody - BSA Free

Immunohistochemistry-Paraffin: Triadin Antibody [NBP1-87417]

Immunohistochemistry-Paraffin: Triadin Antibody [NBP1-87417]

Immunohistochemistry-Paraffin: Triadin Antibody [NBP1-87417] - Staining in human skeletal muscle and skin tissues using anti-TRDN antibody. Corresponding TRDN RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Triadin Antibody [NBP1-87417]

Immunocytochemistry/ Immunofluorescence: Triadin Antibody [NBP1-87417]

Immunocytochemistry/Immunofluorescence: Triadin Antibody [NBP1-87417] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Triadin Antibody [NBP1-87417]

Immunohistochemistry-Paraffin: Triadin Antibody [NBP1-87417]

Immunohistochemistry-Paraffin: Triadin Antibody [NBP1-87417] - Staining of human skeletal muscle shows high expression.
Immunohistochemistry-Paraffin: Triadin Antibody [NBP1-87417]

Immunohistochemistry-Paraffin: Triadin Antibody [NBP1-87417]

Immunohistochemistry-Paraffin: Triadin Antibody [NBP1-87417] - Staining of human skin shows low expression as expected.

Applications for Triadin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Triadin

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR®s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR®s), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, Triadin (MW 95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR®s and RyR®s. Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RYR at the junctional face of the terminal cisternae.

Alternate Names

dJ166D18.1 (triadin), TDN, triadin, TRISK

Gene Symbol

TRDN

Additional Triadin Products

Product Documents for Triadin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Triadin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Triadin Antibody - BSA Free

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Protocols

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