TRIB3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-82067

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

TRIB3 antibody was raised against a 17 amino acid peptide near the center of human TRIB3. The immunogen is located within amino acids 100 - 150 of TRIB3.

Specificity

TRIB3 antibody is human, mouse and rat reactive. At least two isoforms of TRIB3 are known to exist; this antibody will detect both isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRIB3 Antibody - BSA Free

Western Blot: TRIB3 AntibodyBSA Free [NBP2-82067]

Western Blot: TRIB3 AntibodyBSA Free [NBP2-82067]

Western Blot: TRIB3 Antibody [NBP2-82067] - Analysis in mouse kidney tissue lysate (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: TRIB3 Antibody - BSA Free [NBP2-82067]

Immunocytochemistry/ Immunofluorescence: TRIB3 Antibody - BSA Free [NBP2-82067]

Immunocytochemistry/Immunofluorescence: TRIB3 Antibody [NBP2-82067] - Mouse liver tissue 20 ug/ml.
TRIB3 Antibody - BSA Free

Immunohistochemistry: TRIB3 Antibody - BSA Free [NBP2-82067] -

Immunohistochemistry: TRIB3 Antibody - BSA Free [NBP2-82067] - Immunohistochemistry of TRIB3 in mouse liver tissue with TRIB3 antibody at 5 ug/mL.
TRIB3 Antibody - BSA Free

Western Blot: TRIB3 Antibody - BSA Free [NBP2-82067] -

Conditioned medium from OC cells induces CAAs formation via upregulating TRIB3 to suppress phosphorylation of CEBP beta.A Heatmap of differentially expressed genes (DEGs) that are involved in negative regulation of adipocyte differentiation between control adipocytes and CAAs. B Western blot analysis for protein expression levels of TRIB3, DDIT3, and FLCN in adipocytes with treatment as indicated. C Oil red O staining of adipocytes with treatment as indicated. D Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. E ELISA analysis for concentrations of IL-1 beta and IL-6 in the medium of adipocytes with treatment as indicated. F Oil red O staining of adipocytes with treatment as indicated. G Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. H ELISA analysis for concentrations of IL-1 beta and IL-6 in the medium of adipocytes with treatment as indicated. I Western blot analysis for protein expression levels of TRIB3, CEBP beta, and p-CEBP beta in adipocytes with treatment as indicated. J Co-IP assay was performed with anti-TRIB3 or anti-CEBP beta antibodies using extracts prepared from CAAs. The presence of TRIB3 or CEBP beta in IPs was evaluated by immunoblotting (WB). K Oil red O staining of adipocytes with treatment as indicated. L Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. Scale bar = 100 um. Data were expressed as mean +/- SEM of three independent experiments. Student’s t test was used for analysis of the data in C, E, F, H and K. *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41419-024-07311-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TRIB3 Antibody - BSA Free

Western Blot: TRIB3 Antibody - BSA Free [NBP2-82067] -

Conditioned medium from OC cells induces CAAs formation via upregulating TRIB3 to suppress phosphorylation of CEBP beta.A Heatmap of differentially expressed genes (DEGs) that are involved in negative regulation of adipocyte differentiation between control adipocytes and CAAs. B Western blot analysis for protein expression levels of TRIB3, DDIT3, and FLCN in adipocytes with treatment as indicated. C Oil red O staining of adipocytes with treatment as indicated. D Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. E ELISA analysis for concentrations of IL-1 beta and IL-6 in the medium of adipocytes with treatment as indicated. F Oil red O staining of adipocytes with treatment as indicated. G Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. H ELISA analysis for concentrations of IL-1 beta and IL-6 in the medium of adipocytes with treatment as indicated. I Western blot analysis for protein expression levels of TRIB3, CEBP beta, and p-CEBP beta in adipocytes with treatment as indicated. J Co-IP assay was performed with anti-TRIB3 or anti-CEBP beta antibodies using extracts prepared from CAAs. The presence of TRIB3 or CEBP beta in IPs was evaluated by immunoblotting (WB). K Oil red O staining of adipocytes with treatment as indicated. L Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. Scale bar = 100 um. Data were expressed as mean +/- SEM of three independent experiments. Student’s t test was used for analysis of the data in C, E, F, H and K. *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41419-024-07311-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TRIB3 Antibody - BSA Free

Western Blot: TRIB3 Antibody - BSA Free [NBP2-82067] -

Conditioned medium from OC cells induces CAAs formation via upregulating TRIB3 to suppress phosphorylation of CEBP beta.A Heatmap of differentially expressed genes (DEGs) that are involved in negative regulation of adipocyte differentiation between control adipocytes and CAAs. B Western blot analysis for protein expression levels of TRIB3, DDIT3, and FLCN in adipocytes with treatment as indicated. C Oil red O staining of adipocytes with treatment as indicated. D Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. E ELISA analysis for concentrations of IL-1 beta and IL-6 in the medium of adipocytes with treatment as indicated. F Oil red O staining of adipocytes with treatment as indicated. G Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. H ELISA analysis for concentrations of IL-1 beta and IL-6 in the medium of adipocytes with treatment as indicated. I Western blot analysis for protein expression levels of TRIB3, CEBP beta, and p-CEBP beta in adipocytes with treatment as indicated. J Co-IP assay was performed with anti-TRIB3 or anti-CEBP beta antibodies using extracts prepared from CAAs. The presence of TRIB3 or CEBP beta in IPs was evaluated by immunoblotting (WB). K Oil red O staining of adipocytes with treatment as indicated. L Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. Scale bar = 100 um. Data were expressed as mean +/- SEM of three independent experiments. Student’s t test was used for analysis of the data in C, E, F, H and K. *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41419-024-07311-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TRIB3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIB3

TRIB3 is encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1.

Alternate Names

C20orf97, dJ1103G7.3, neuronal cell death inducible putative kinase, Neuronal cell death-inducible putative kinase, NIPK, p65-interacting inhibitor of NF-kappaB, p65-interacting inhibitor of NF-kappa-B, SINK, SKIP3, TRB-3, TRB3chromosome 20 open reading frame 97, tribbles homolog 3, tribbles homolog 3 (Drosophila)

Gene Symbol

TRIB3

Additional TRIB3 Products

Product Documents for TRIB3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TRIB3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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