TRIB3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-82067
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
TRIB3 antibody was raised against a 17 amino acid peptide near the center of human TRIB3. The immunogen is located within amino acids 100 - 150 of TRIB3.
Specificity
TRIB3 antibody is human, mouse and rat reactive. At least two isoforms of TRIB3 are known to exist; this antibody will detect both isoforms.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TRIB3 Antibody - BSA Free
Western Blot: TRIB3 AntibodyBSA Free [NBP2-82067]
Western Blot: TRIB3 Antibody [NBP2-82067] - Analysis in mouse kidney tissue lysate (A) 1 and (B) 2 ug/ml.Immunocytochemistry/ Immunofluorescence: TRIB3 Antibody - BSA Free [NBP2-82067]
Immunocytochemistry/Immunofluorescence: TRIB3 Antibody [NBP2-82067] - Mouse liver tissue 20 ug/ml.Immunohistochemistry: TRIB3 Antibody - BSA Free [NBP2-82067] -
Immunohistochemistry: TRIB3 Antibody - BSA Free [NBP2-82067] - Immunohistochemistry of TRIB3 in mouse liver tissue with TRIB3 antibody at 5 ug/mL.Western Blot: TRIB3 Antibody - BSA Free [NBP2-82067] -
Conditioned medium from OC cells induces CAAs formation via upregulating TRIB3 to suppress phosphorylation of CEBP beta.A Heatmap of differentially expressed genes (DEGs) that are involved in negative regulation of adipocyte differentiation between control adipocytes and CAAs. B Western blot analysis for protein expression levels of TRIB3, DDIT3, and FLCN in adipocytes with treatment as indicated. C Oil red O staining of adipocytes with treatment as indicated. D Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. E ELISA analysis for concentrations of IL-1 beta and IL-6 in the medium of adipocytes with treatment as indicated. F Oil red O staining of adipocytes with treatment as indicated. G Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. H ELISA analysis for concentrations of IL-1 beta and IL-6 in the medium of adipocytes with treatment as indicated. I Western blot analysis for protein expression levels of TRIB3, CEBP beta, and p-CEBP beta in adipocytes with treatment as indicated. J Co-IP assay was performed with anti-TRIB3 or anti-CEBP beta antibodies using extracts prepared from CAAs. The presence of TRIB3 or CEBP beta in IPs was evaluated by immunoblotting (WB). K Oil red O staining of adipocytes with treatment as indicated. L Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. Scale bar = 100 um. Data were expressed as mean +/- SEM of three independent experiments. Student’s t test was used for analysis of the data in C, E, F, H and K. *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41419-024-07311-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: TRIB3 Antibody - BSA Free [NBP2-82067] -
Conditioned medium from OC cells induces CAAs formation via upregulating TRIB3 to suppress phosphorylation of CEBP beta.A Heatmap of differentially expressed genes (DEGs) that are involved in negative regulation of adipocyte differentiation between control adipocytes and CAAs. B Western blot analysis for protein expression levels of TRIB3, DDIT3, and FLCN in adipocytes with treatment as indicated. C Oil red O staining of adipocytes with treatment as indicated. D Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. E ELISA analysis for concentrations of IL-1 beta and IL-6 in the medium of adipocytes with treatment as indicated. F Oil red O staining of adipocytes with treatment as indicated. G Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. H ELISA analysis for concentrations of IL-1 beta and IL-6 in the medium of adipocytes with treatment as indicated. I Western blot analysis for protein expression levels of TRIB3, CEBP beta, and p-CEBP beta in adipocytes with treatment as indicated. J Co-IP assay was performed with anti-TRIB3 or anti-CEBP beta antibodies using extracts prepared from CAAs. The presence of TRIB3 or CEBP beta in IPs was evaluated by immunoblotting (WB). K Oil red O staining of adipocytes with treatment as indicated. L Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. Scale bar = 100 um. Data were expressed as mean +/- SEM of three independent experiments. Student’s t test was used for analysis of the data in C, E, F, H and K. *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41419-024-07311-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: TRIB3 Antibody - BSA Free [NBP2-82067] -
Conditioned medium from OC cells induces CAAs formation via upregulating TRIB3 to suppress phosphorylation of CEBP beta.A Heatmap of differentially expressed genes (DEGs) that are involved in negative regulation of adipocyte differentiation between control adipocytes and CAAs. B Western blot analysis for protein expression levels of TRIB3, DDIT3, and FLCN in adipocytes with treatment as indicated. C Oil red O staining of adipocytes with treatment as indicated. D Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. E ELISA analysis for concentrations of IL-1 beta and IL-6 in the medium of adipocytes with treatment as indicated. F Oil red O staining of adipocytes with treatment as indicated. G Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. H ELISA analysis for concentrations of IL-1 beta and IL-6 in the medium of adipocytes with treatment as indicated. I Western blot analysis for protein expression levels of TRIB3, CEBP beta, and p-CEBP beta in adipocytes with treatment as indicated. J Co-IP assay was performed with anti-TRIB3 or anti-CEBP beta antibodies using extracts prepared from CAAs. The presence of TRIB3 or CEBP beta in IPs was evaluated by immunoblotting (WB). K Oil red O staining of adipocytes with treatment as indicated. L Western blot analysis for protein expression levels of PPAR gamma, CEBP alpha and APN in adipocytes with treatment as indicated. Scale bar = 100 um. Data were expressed as mean +/- SEM of three independent experiments. Student’s t test was used for analysis of the data in C, E, F, H and K. *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41419-024-07311-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for TRIB3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRIB3
Alternate Names
C20orf97, dJ1103G7.3, neuronal cell death inducible putative kinase, Neuronal cell death-inducible putative kinase, NIPK, p65-interacting inhibitor of NF-kappaB, p65-interacting inhibitor of NF-kappa-B, SINK, SKIP3, TRB-3, TRB3chromosome 20 open reading frame 97, tribbles homolog 3, tribbles homolog 3 (Drosophila)
Gene Symbol
TRIB3
Additional TRIB3 Products
Product Documents for TRIB3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TRIB3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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