TRIM21/SSA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87122

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRIM21 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIM21/SSA1 Antibody - BSA Free

Western Blot: TRIM21/SSA1 Antibody [NBP1-87122]

Western Blot: TRIM21/SSA1 Antibody [NBP1-87122]

Western Blot: TRIM21/SSA1 Antibody [NBP1-87122] - Analysis in control (vector only transfected HEK293T lysate) and TRIM21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TRIM21/SSA1 Antibody [NBP1-87122]

Immunocytochemistry/ Immunofluorescence: TRIM21/SSA1 Antibody [NBP1-87122]

Immunocytochemistry/Immunofluorescence: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human cell line HUVEC TERT2 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122]

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122]

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in lymphoid cells.
Western Blot: TRIM21/SSA1 Antibody [NBP1-87122]

Western Blot: TRIM21/SSA1 Antibody [NBP1-87122]

Western Blot: TRIM21/SSA1 Antibody [NBP1-87122] - Analysis in human cell line CAPAN-2.
Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122]

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122]

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human kidney shows moderate cytoplasmic and stronger nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122]

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122]

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human placenta shows strong cytoplasmic immunoreactivity in monocytes, as well as moderate positivity in trophoblast.
Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122]

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122]

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human skeletal muscle shows only weak positivity in striated muscle fibers as expected.

Applications for TRIM21/SSA1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM21

SSA1 encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

Long Name

Tripartite Motif-containing Protein 21

Alternate Names

RNF81, Ro52, SSA1

Gene Symbol

TRIM21

Additional TRIM21 Products

Product Documents for TRIM21/SSA1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRIM21/SSA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRIM21/SSA1 Antibody - BSA Free

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Protocols

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