TRIM32 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13484

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRIM32 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: NLTPLSVAMNCQGLIGVTDSYDNSLKVYTLDGHCVACHRSQLSKPWGITALPSGQFVVTDVEGGKLWCFTVDRGSGVVKYSCLCSAVRPKFVTCDA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit TRIM32 Antibody - BSA Free (NBP2-13484) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TRIM32 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TRIM32 Antibody [NBP2-13484]

Immunocytochemistry/ Immunofluorescence: TRIM32 Antibody [NBP2-13484]

Immunocytochemistry/Immunofluorescence: TRIM32 Antibody [NBP2-13484] - Immunofluorescent staining of human cell line A-431 shows localization to intermediate filaments.
Immunohistochemistry-Paraffin: TRIM32 Antibody [NBP2-13484]

Immunohistochemistry-Paraffin: TRIM32 Antibody [NBP2-13484]

Immunohistochemistry-Paraffin: TRIM32 Antibody [NBP2-13484] - Staining of human gallbladder shows strong nuclear and cytoplasmic positivity in glandular cells.
TRIM32 Antibody - BSA Free Western Blot: TRIM32 Antibody - BSA Free [NBP2-13484]

Western Blot: TRIM32 Antibody - BSA Free [NBP2-13484]

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Lane 5: Human liver tissue
TRIM32 Antibody - BSA Free Immunohistochemistry: TRIM32 Antibody - BSA Free [NBP2-13484]

Immunohistochemistry: TRIM32 Antibody - BSA Free [NBP2-13484]

Staining of human prostate shows strong nuclear positivity in glandular cells.
TRIM32 Antibody - BSA Free Immunohistochemistry: TRIM32 Antibody - BSA Free [NBP2-13484]

Immunohistochemistry: TRIM32 Antibody - BSA Free [NBP2-13484]

Staining of human small intestine shows strong nuclear and cytoplasmic positivity in glandular cells.
TRIM32 Antibody - BSA Free Immunohistochemistry: TRIM32 Antibody - BSA Free [NBP2-13484]

Immunohistochemistry: TRIM32 Antibody - BSA Free [NBP2-13484]

Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
TRIM32 Antibody - BSA Free Immunohistochemistry: TRIM32 Antibody - BSA Free [NBP2-13484]

Immunohistochemistry: TRIM32 Antibody - BSA Free [NBP2-13484]

Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.

Applications for TRIM32 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM32

TRIM32 is encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.

Long Name

Tripartite Motif-containing Protein 32

Alternate Names

BBS11, HT2A, LGMD2H, TATIP

Gene Symbol

TRIM32

Additional TRIM32 Products

Product Documents for TRIM32 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRIM32 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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