TRIM72 Antibody (HL1853) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25729

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rabbit (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1853 expressed in HEK293

Format

Azide and BSA Free
View all TRIM72 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human TRIM72.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIM72 Antibody (HL1853) - Azide and BSA Free

TRIM72 Antibody (HL1853) - Azide and BSA Free

Immunohistochemistry-Paraffin: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] -

Immunohistochemistry-Paraffin: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] - TRIM72 antibody [HL1853] detects TRIM72 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. TRIM72 stained by TRIM72 antibody [HL1853] (NBP3-25729) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TRIM72 Antibody (HL1853) - Azide and BSA Free

Immunohistochemistry-Paraffin: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] -

Immunohistochemistry-Paraffin: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] - TRIM72 antibody [HL1853] detects TRIM72 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. TRIM72 stained by TRIM72 antibody [HL1853] (NBP3-25729) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TRIM72 Antibody (HL1853) - Azide and BSA Free

Western Blot: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] -

Western Blot: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] - Various whole cell extracts (10 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TRIM72 antibody [HL1853] (NBP3-25729) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
TRIM72 Antibody (HL1853) - Azide and BSA Free

Western Blot: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] -

Western Blot: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TRIM72 antibody [HL1853] (NBP3-25729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
TRIM72 Antibody (HL1853) - Azide and BSA Free

Western Blot: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] -

Western Blot: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with TRIM72 antibody [HL1853] (NBP3-25729) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
TRIM72 Antibody (HL1853) - Azide and BSA Free

Western Blot: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] -

Western Blot: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM72 antibody [HL1853] (NBP3-25729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for TRIM72 Antibody (HL1853) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM72

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of therepair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membranedamage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization atthe injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to theinjury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealingprocess. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membranebudding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (Bysimilarity)

Alternate Names

MG53Mg53, Mitsugumin-53, tripartite motif containing 72, tripartite motif-containing 72, tripartite motif-containing protein 72

Gene Symbol

TRIM72

Additional TRIM72 Products

Product Documents for TRIM72 Antibody (HL1853) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TRIM72 Antibody (HL1853) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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