TrkA Antibody (JJ084-04)

Novus Biologicals | Catalog # NBP2-67473

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JJ084-04 expressed in HEK293
View all TrkA Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human TrkA aa 747-796 / 796. (SwissProt: P04629 Human; SwissProt: Q3UFB7 Mouse; SwissProt: P35739 Rat)

Localization

Cell membrane, Early endosome membrane, Late endosome membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TrkA Antibody (JJ084-04)

Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473]

Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473]

Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473]

Immunocytochemistry/ Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473]

Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473]

Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473]

Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded mouse liver tissue using anti-TrkA antibody. Counter stained with hematoxylin.
Flow Cytometry: TrkA Antibody (JJ084-04) [NBP2-67473]

Flow Cytometry: TrkA Antibody (JJ084-04) [NBP2-67473]

Flow Cytometry: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of SH-SY-5Y cells with TrkA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473]

Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473]

Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on different lysates using anti-TrkA antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: SH-SY-5Y
Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473]

Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473]

Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Western blot analysis of TrkA on N2A cell lysates using anti-TrkA antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473]

Immunocytochemistry/ Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473]

Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473]

Immunocytochemistry/ Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473]

Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473]

Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473]

Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded human tonsil tissue using anti-TrkA antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473]

Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473]

Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded mouse kidney tissue using anti-TrkA antibody. Counter stained with hematoxylin.

Applications for TrkA Antibody (JJ084-04)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TrkA

The Trk proto-oncogene encodes a 140 kDa, membrane-spanning protein tyrosine kinase that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Trk A was found to be expressed in the nervous system and phosphorylated in response to NGF (Nerve Growth Factor). Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes (2). Trk A is expressed in neural and nonneuronal tissues. Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC) (3).

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Alternate Names

NTRK-1, NTRK1

Gene Symbol

NTRK1

Additional TrkA Products

Product Documents for TrkA Antibody (JJ084-04)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TrkA Antibody (JJ084-04)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Receptor Tyrosine Kinases (RTKs)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TrkA Antibody (JJ084-04)

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  • Q: I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.

    A:

    NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

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