TRMT12 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76583

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRMT12 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TYW2. The immunogen is located within the first 50 amino acids of TYW2.

Specificity

TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRMT12 Antibody - BSA Free

Western Blot: TRMT12 AntibodyBSA Free [NBP1-76583]

Western Blot: TRMT12 AntibodyBSA Free [NBP1-76583]

Western Blot: TRMT12 Antibody [NBP1-76583] - Western blot analysis of TYW2 in K562 cell lysate with TYW2 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.
TRMT12 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TRMT12 Antibody - BSA Free [NBP1-76583] -

Immunocytochemistry/ Immunofluorescence: TRMT12 Antibody - BSA Free [NBP1-76583] - Immunocytochemistry of TRMT12 in K562 cells with TRMT12 antibody at 10 ug/mL.

Applications for TRMT12 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Western Blot

0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TRMT12

TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.

Alternate Names

Alpha-amino-alpha-carboxypropyl transferase TYW2, EC 2.1.1, EC 2.1.1.32, EC 2.5.1.-, FLJ20772, homolog of yeast tRNA methyltransferase, TRM12, tRNA methyltranferase 12 homolog, tRNA methyltranferase 12 homolog (S. cerevisiae), tRNA methyltransferase 12 homolog (S. cerevisiae), tRNA-yW synthesizing protein 2, tRNA-yW-synthesizing protein 2, TYW2tRNA wybutosine-synthesizing protein 2 homolog

Gene Symbol

TRMT12

UniProt

Q53H54 (Human)

Additional TRMT12 Products

Product Documents for TRMT12 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRMT12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRMT12 Antibody - BSA Free

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Protocols

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