Tropomyosin-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33716

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Tropomyosin-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tropomyosin-1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (80%), Xenopus laevis (83%), Rabbit (88%), Chicken (82%), Sheep (88%), Bovine (88%).

Localization

Cytoplasm, cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tropomyosin-1 Antibody - BSA Free

Western Blot: Tropomyosin-1 Antibody [NBP1-33716]

Western Blot: Tropomyosin-1 Antibody [NBP1-33716]

Western Blot: Tropomyosin-1 Antibody [NBP1-33716] - A. 50 ug rat muscle lysate/extract 10 % SDS-PAGETropomyosin 1 antibody dilution: 1:6000
Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody [NBP1-33716]

Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody [NBP1-33716]

Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody [NBP1-33716] - Paraffin-embedded mouse vein. Tropomyosin 1 antibody dilution: 1:500.
Western Blot: Tropomyosin-1 Antibody [NBP1-33716]

Western Blot: Tropomyosin-1 Antibody [NBP1-33716]

Western Blot: Tropomyosin-1 Antibody [NBP1-33716] - Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody [NBP1-33716]

Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody [NBP1-33716]

Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody [NBP1-33716] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody [NBP1-33716]

Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody [NBP1-33716]

Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody [NBP1-33716] - Paraffin-embedded mouse muscle. Tropomyosin 1 antibody dilution: 1:500
Tropomyosin-1 Antibody

Immunocytochemistry/ Immunofluorescence: Tropomyosin-1 Antibody [NBP1-33716] -

Immunocytochemistry/ Immunofluorescence: Tropomyosin-1 Antibody [NBP1-33716] - Tropomyosin-1 antibody detects Tropomyosin-1 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tropomyosin-1 stained by Tropomyosin-1 antibody (NBP1-33716) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Scale bar= 10 um.
Tropomyosin-1 Antibody

Western Blot: Tropomyosin-1 Antibody [NBP1-33716] -

Western Blot: Tropomyosin-1 Antibody [NBP1-33716] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Tropomyosin-1 antibody (NBP1-33716) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Tropomyosin-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Tropomyosin-1

Tropomyosin is a rigid rod shaped protein closely associated with actin filaments. Non-muscle forms of tropomyosin have been identified in a wide range of cell types.

Alternate Names

alpha tropomyosin, Alpha-tropomyosin, C15orf13, cardiomyopathy, hypertrophic 3, chromosome 15 open reading frame 13, CMD1Y, CMH3, HTM-alpha, sarcomeric tropomyosin kappa, TMSA, tropomyosin 1 (alpha), tropomyosin 1 (alpha) isoform 1, tropomyosin 1 (alpha) isoform 2, tropomyosin 1 (alpha) isoform 3, tropomyosin 1 (alpha) isoform 4, tropomyosin 1 (alpha) isoform 5, tropomyosin 1 (alpha) isoform 6, tropomyosin 1 (alpha) isoform 7, tropomyosin alpha-1 chain, tropomyosin-1

Gene Symbol

TPM1

Additional Tropomyosin-1 Products

Product Documents for Tropomyosin-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Tropomyosin-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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