Troponin I Type 3 (cardiac) Antibody (1E7) - Azide and BSA Free

Novus Biologicals | Catalog # H00007137-M04

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E7

Format

Azide and BSA Free
View all Troponin I/TNNI3 Primary Antibodies »

Product Specifications

Immunogen

TNNI3 (NP_000354, 102 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES

Specificity

TNNI3 - troponin I type 3 (cardiac)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Troponin I Type 3 (cardiac) Antibody (1E7) - Azide and BSA Free

Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]

Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]

Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04] - Analysis of TNNI3 expression in transfected 293T cell line by TNNI3 monoclonal antibody (M04), clone 1E7. Lane 1: TNNI3 transfected lysatE (24 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]

Immunocytochemistry/ Immunofluorescence: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]

Immunocytochemistry/Immunofluorescence: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04] - Analysis of monoclonal antibody to TNNI3 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04] - Analysis of monoclonal antibody to TNNI3 on formalin-fixed paraffin-embedded human heart. Antibody concentration 0.7 ug/ml
Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]

Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]

Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04] - Western blot analysis of TNNI3 over-expressed 293 cell line, cotransfected with TNNI3 Validated Chimera RNAi or non-transfected control. Blot probed with H00007137-M04. GAPDH (36.1 kDa) used as loading control.
ELISA: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]

ELISA: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]

ELISA: Troponin I Type 3 (cardiac) Antibody (1E7.) [H00007137-M04] - Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04] - Detection limit for recombinant GST tagged TNNI3 is 1 ng/ml as a capture antibody.

Applications for Troponin I Type 3 (cardiac) Antibody (1E7) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi validaton, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Troponin I/TNNI3

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Entrez Gene IDs

7137 (Human)

Gene Symbol

TNNI3

OMIM

115210 (Human)

UniProt

NP_000354 (Human)

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) Antibody (1E7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Troponin I Type 3 (cardiac) Antibody (1E7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Troponin I Type 3 (cardiac) Antibody (1E7) - Azide and BSA Free

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Protocols

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FAQs

No product specific FAQs exist for this product.

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