Troponin T Type 2 (cardiac) Antibody (5-C7)
Novus Biologicals | Catalog # NBP2-67211
![Western Blot: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211]](https://resources.rndsystems.com/images/products/Troponin-T-Type-2-cardiac-Antibody-5-C7-Western-Blot-NBP2-67211-img0001.jpg "Western Blot: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 5-C7
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Product Specifications
Immunogen
Synthetic peptide within human Troponin T Type 2 (cardiac) 50-100 aa. (SwissProt: P45379 Human; SwissProt: P50752 Mouse)
Reactivity Notes
Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.
Localization
Cytoskeleton. Cytosol.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Troponin T Type 2 (cardiac) Antibody (5-C7)
Western Blot: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211]
Western Blot: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211] - Analysis on human heart lysate using anti-TNNT2 Mouse mAb.![Immunocytochemistry/ Immunofluorescence: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211]](https://resources.rndsystems.com/images/products/Troponin-T-Type-2-cardiac-Antibody-5-C7-Immunocytochemistry-Immunofluorescence-NBP2-67211-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211]")
Immunocytochemistry/ Immunofluorescence: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211]
Immunocytochemistry/Immunofluorescence: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211] - ICC staining Troponin T Type 2 (cardiac) (red) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211]](https://resources.rndsystems.com/images/products/Troponin-T-Type-2-cardiac-Antibody-5-C7-Immunohistochemistry-Paraffin-NBP2-67211-img0004.jpg "Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211]")
Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211]
Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211] - Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Troponin T Type 2 (cardiac) antibody. Counter stained with hematoxylin.![Flow Cytometry: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211]](https://resources.rndsystems.com/images/products/Troponin-T-Type-2-cardiac-Antibody-5-C7-Flow-Cytometry-NBP2-67211-img0002.jpg "Flow Cytometry: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211]")
Flow Cytometry: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211]
Flow Cytometry: Troponin T Type 2 (cardiac) Antibody (5-C7) [NBP2-67211] - Flow cytometric analysis of SK-Br-3 cells with Troponin T Type 2 (cardiac) antibody at 1/100 dilution (blue) compared with an unlabeled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.Applications for Troponin T Type 2 (cardiac) Antibody (5-C7)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:2000-1:5000
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH7.4), 0.2% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Troponin T Type 2 (cardiac)
Alternate Names
cardiac, CMD1D, CMPD2, dilated 1D (autosomal dominant), hypertrophic 2, MGC3889, RCM3, TnTc, troponin T type 2 (cardiac), troponin T, cardiac muscle
Gene Symbol
TNNT2
Additional Troponin T Type 2 (cardiac) Products
Product Documents for Troponin T Type 2 (cardiac) Antibody (5-C7)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Troponin T Type 2 (cardiac) Antibody (5-C7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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