TRPC6 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-77260
![Western Blot: TRPC6 AntibodyBSA Free [NBP1-77260]](https://resources.rndsystems.com/images/products/TRPC6-Antibody-Western-Blot-NBP1-77260-img0002.jpg "Western Blot: TRPC6 AntibodyBSA Free [NBP1-77260]")
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Format
Product Specifications
Immunogen
Specificity
Clonality
Host
Isotype
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TRPC6 Antibody - BSA Free
Western Blot: TRPC6 AntibodyBSA Free [NBP1-77260]
Western Blot: TRPC6 Antibody [NBP1-77260] - Mouse lung tissue lysate with TRPC6 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.![Immunohistochemistry-Paraffin: TRPC6 Antibody - BSA Free [NBP1-77260]](https://resources.rndsystems.com/images/products/TRPC6-Antibody-Immunohistochemistry-Paraffin-NBP1-77260-img0010.jpg "Immunohistochemistry-Paraffin: TRPC6 Antibody - BSA Free [NBP1-77260]")
Immunohistochemistry-Paraffin: TRPC6 Antibody - BSA Free [NBP1-77260]
Immunohistochemistry-Paraffin: TRPC6 Antibody [NBP1-77260] - Staining of TRPC6 in mouse lung tissue with TRPC6 antibody at 5 ug/ml.
Immunohistochemistry: TRPC6 Antibody - BSA Free [NBP1-77260] -
Immunohistochemistry: TRPC6 Antibody - BSA Free [NBP1-77260] - Immunohistochemistry of TRPC6 in mouse lung tissue with TRPC6 antibody at 10 u/mL.
Immunocytochemistry/ Immunofluorescence: TRPC6 Antibody - BSA Free [NBP1-77260] -
Immunocytochemistry/ Immunofluorescence: TRPC6 Antibody - BSA Free [NBP1-77260] - Immunofluorescence of TRPC6 in Mouse Lung cells with TRPC6 antibody at 20 ug/mL.Applications for TRPC6 Antibody - BSA Free
ELISA
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Background: TRPC6
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional TRPC6 Products
Product Documents for TRPC6 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for TRPC6 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for TRPC6 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for TRPC6 Antibody - BSA Free
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Q: I have tested this antibody and would like to share the results from my first preliminary experiment. This Ab has been tested in ICC using a human cell line. Blocking buffer contains either goat or donkey serum. Dilutions: both 1:1000 and 1500 have been tested. Secondary ab is goat anti rabbit FITC (1:400). Results: every single cell shows VERY WEAK signal around only the cell body. No signal was detected in any cytoplasmic processes, which is really different from TRPM2. As the signal is weak, I will increase the concentration by using the following dilutions instead: 1: 200 and 1:500 to find out if the staining is getting stronger. Questions: Despite the weak staining, every single cell has been stained. Thus, (1) How can I confirm that the staining is specific? (2) How can confirm the staining is in the cell membrane?
A: Membrane staining should be around the edge of the cell. If this is not what you are seeing then I would suspect that your staining is unsuccessful. You may need to permeabilize since this antibody is expected to stain the intracellular portion of TRPC6 and what you're seeing may simply be background and non-specific. Once again, confirmation of staining should best be discussed with your mentor who should have greater knowledge on your proteins of interest than I do.