TRPM2 Antibody - BSA Free
Novus Biologicals | Catalog # NB500-242
![Western Blot: TRPM2 Antibody [NB500-242]](https://resources.rndsystems.com/images/products/TRPM2-Antibody-Western-Blot-NB500-242-img0001.jpg "Western Blot: TRPM2 Antibody [NB500-242]")
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Species Reactivity
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Scientific Data Images for TRPM2 Antibody - BSA Free
Western Blot: TRPM2 Antibody [NB500-242]
Western Blot: TRPM2 Antibody [NB500-242] - Samples: Jurkat Membrane Prep (50, 15, 5 ug). Antibody: Affinity purified rabbit anti-TRPM2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: TRPM2 Antibody [NB500-242]](https://resources.rndsystems.com/images/products/TRPM2-Antibody-Immunohistochemistry-NB500-242-img0002.jpg "Immunoprecipitation: TRPM2 Antibody [NB500-242]")
Immunoprecipitation: TRPM2 Antibody [NB500-242]
Immunoprecipitation: TRPM2 Antibody [NB500-242] - Samples: Membrane Prep (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPM2 antibody NB500-242 used for IP at 6 ug per reaction. TRPM2 was also immunoprecipitated by rabbit anti-TRPM2 antibody NB500-241. For blotting immunoprecipitated TRPM2, NB500-242 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for TRPM2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Frozen
Immunoprecipitation
Western Blot
Formulation, Preparation, and Storage
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Background: TRPM2
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional TRPM2 Products
Product Documents for TRPM2 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for TRPM2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for TRPM2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for TRPM2 Antibody - BSA Free
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Q: I have tested this antibody and would like to share the results from my first preliminary experiment. This Ab has been tested in ICC using a human cell line. Blocking buffer contains either goat or donkey serum. Dilutions: 1:50 and 1: 100 have been tested. Secondary Ab is goat anti rabbit FITC (1:400). Control test: No signal is detected when TRPC4 is omitted. Results: a bright staining around the entire cell structure including the cell membrane has been detected in every single cell. Questions: (1) Is an absorption test absolutely required before I can confidently say the staining is specific? If yes, what's your suggested protocol? (2) How can confirm the staining is in the cell membrane?
A: TRPM2 is expected to be expressed on the cell membrane. It sounds to me like you are seeing membrane-bound staining so it appears to be working just fine. As far as whether or not you need to do an absorption test and whether or not you believe that the staining is truly in the cell membrane, you may want to consult your mentor as they should be able to interpret data on these proteins better than I can.