TRPM4 Antibody (OTI10H5)

Novus Biologicals | Catalog # NBP1-48036

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI10H5
View all TRPM4 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TRPM4 (NP_060106) produced in HEK293T cell.

Specificity

This antibody is specific for Homo sapiens transient receptor potential cation channel, subfamily M, member 4 (TRPM4).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

134.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRPM4 Antibody (OTI10H5)

Western Blot: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Western Blot: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Western Blot: TRPM4 Antibody (OTI10H5) [NBP1-48036] - Analysis of extracts (35ug) from 9 different cell lines by using anti-TRPM4 monoclonal antibody.
Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036] - Human endometrial carcinoma tissue section. Fixed paraffin embedded sections were deparaffinized. EDTA was used for antigen retrieval. Samples were incubated O/N in 4 C with the primary antibody (1:200 dilution). IHC-P image submitted by a verified review.
Western Blot: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Western Blot: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Western Blot: TRPM4 Antibody (10H5) [NBP1-48036] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRPM4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRPM4.
Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (10H5) [NBP1-48036] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRPM4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (10H5) [NBP1-48036] - Staining of paraffin-embedded Human Kidney tissue using anti-TRPM4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (10H5) [NBP1-48036] - Staining of paraffin-embedded Human liver tissue using anti-TRPM4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (10H5) [NBP1-48036] - Staining of paraffin-embedded Human Ovary tissue using anti-TRPM4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (10H5) [NBP1-48036] - Staining of paraffin-embedded Human pancreas tissue using anti-TRPM4 mouse monoclonal antibody.
Immunohistochemistry: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry: TRPM4 Antibody (OTI10H5) [NBP1-48036] - Human heart tissue was blocked with 10% serum and stained with the primary antibody at 1:100 dilution overnight at 4 degrees. A secondary FITC-conjugated anti-mouse antibody was used in 1:400 dilution for 2 hours at room temperature. IHC image submitted by a verified customer review.
TRPM4 Antibody (OTI10H5)

TRPM4 Antibody (OTI10H5)[NBP1-48036]

TRPM4 Antibody (OTI10H5)[NBP1-48036]Simple Western� analysis of endogenous protein ACTN1 from HELA lysates (0.2 mg/mL) using ACTN1 Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for TRPM4 Antibody (OTI10H5)

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:5000-10000

Reviewed Applications

Read 2 reviews rated 5 using NBP1-48036 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRPM4

Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload

Long Name

Transient receptor potential cation channel subfamily M member 4

Alternate Names

hTRPM4, LTrpC-4, LTrpC4, Melastatin-4

Entrez Gene IDs

54795 (Human)

Gene Symbol

TRPM4

Additional TRPM4 Products

Product Documents for TRPM4 Antibody (OTI10H5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TRPM4 Antibody (OTI10H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TRPM4 Antibody (OTI10H5) (2)

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  • TRPM4 Antibody (OTI10H5)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Endometrial carcinoma
    Species: Human
    Verified Customer | Posted 12/13/2021
    Human endometrial carcinoma tissue
    Fixed paraffin embedded sections were deparaffinized. EDTA was used for antigen retrieval. Samples were incubated O/N in 4 C with the primary antibody (1:200 dilution).
    TRPM4 Antibody (OTI10H5) NBP1-48036
  • TRPM4 Antibody (OTI10H5)
    Name: Anonymous
    Application: IHC/Immunofluorescence
    Sample Tested: Heart tissue
    Species: Human
    Verified Customer | Posted 08/27/2021
    The tissue was blocked with 10% serum and stained with the primary antibody at 1:100 dilution overnight at 4 degrees. A secondary FITC-conjugated anti-mouse antibody was used in 1:400 dilution for 2 hours at room temperature.
    TRPM4 Antibody (OTI10H5) NBP1-48036

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Protocols

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