TTF-1 / NKX2-1 Antibody (6C11) - BSA Free

Novus Biologicals | Catalog # NBP3-26111

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6C11 expressed in HEK293

Format

BSA Free
View all TTF-1/NKX2-1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human TTF-1/NKX2-1 [UniProt P43699]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TTF-1 / NKX2-1 Antibody (6C11) - BSA Free

TTF-1 / NKX2-1 Antibody (6C11)

Immunohistochemistry: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] -

Immunohistochemistry: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] - Image of TTF-1 / NKX2-1 Antibody (6C11) diluted at 1:100 and staining in paraffin-embedded human lung tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
TTF-1 / NKX2-1 Antibody (6C11)

Immunohistochemistry: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] -

Immunohistochemistry: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] - Image of TTF-1 / NKX2-1 Antibody (6C11) diluted at 1:100 and staining in paraffin-embedded human lung tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
TTF-1 / NKX2-1 Antibody (6C11)

Western Blot: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] -

Western Blot: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] - Positive Western Blot detected in: HepG2 whole cell lysate, U251 whole cell lysate, U87 whole cell lysate.
All lanes: TTF-1 / NKX2-1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 39 kDa
Observed band size: 39 kDa
TTF-1 / NKX2-1 Antibody (6C11)

Flow Cytometry: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] -

Flow Cytometry: TTF-1 / NKX2-1 Antibody (6C11) [NBP3-26111] - Overlay histogram showing A549 cells stained with TTF-1 / NKX2-1 Antibody (6C11) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for TTF-1 / NKX2-1 Antibody (6C11) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:20-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: TTF-1/NKX2-1

The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).

Long Name

NK2 Homeobox 1

Alternate Names

BCH, BHC, NKX2-1, NKX2.1, NKX2A, T/EBP, TITF1, TTF1

Gene Symbol

NKX2-1

Additional TTF-1/NKX2-1 Products

Product Documents for TTF-1 / NKX2-1 Antibody (6C11) - BSA Free

Certificate of Analysis

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Product Specific Notices for TTF-1 / NKX2-1 Antibody (6C11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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