Tubulin alpha-8 Antibody (OTI2G6)

Novus Biologicals | Catalog # NBP2-02474

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2G6
View all Tubulin alpha-8 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TUBA8(NP_061816) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tubulin alpha-8 Antibody (OTI2G6)

Western Blot: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Western Blot: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Western Blot: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - WB analysis of extracts (35ug) from 9 different cell lines by using anti-Tubulin alpha-8 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunocytochemistry/ Immunofluorescence: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunocytochemistry/Immunofluorescence: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tubulin alpha-8.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of paraffin-embedded prostate tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Western Blot: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Western Blot: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Western Blot: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tubulin alpha-8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Tubulin alpha-8.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of paraffin-embedded Kidney tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - Staining of paraffin-embedded pancreas tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Immunoprecipitation: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunoprecipitation: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474]

Immunoprecipitation: Tubulin alpha-8 Antibody (OTI2G6) [NBP2-02474] - (Negative control: IP without adding anti-TUBA8 antibody.). For each experiment, 500ul of DDK tagged TUBA8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TUBA8 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Applications for Tubulin alpha-8 Antibody (OTI2G6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50-1:150

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.82 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tubulin alpha-8

Microtubules are cylindrical tubes of 20-25 nm in diameter. They are composed of protofilaments which are in turn composed of alpha- and beta-tubulin polymers. Each microtubule is polarized, at one end alpha-subunits are exposed (-) and at the other beta-subunits are exposed (+). Microtubules act as a scaffold to determine cell shape, and provide a backbone for cell organelles and vesicles to move on, a process that requires motor proteins. The major microtubule motor proteins are kinesin, which generally moves towards the (+) end of the microtubule, and dynein, which generally moves towards the (-) end. Microtubules also form the spindle fibers for separating chromosomes during mitosis.

Long Name

Tubulin alpha-8 chain

Alternate Names

Alpha-tubulin 8, TUBA8, TUBAL2

Entrez Gene IDs

51807 (Human)

Gene Symbol

TUBA8

Additional Tubulin alpha-8 Products

Product Documents for Tubulin alpha-8 Antibody (OTI2G6)

Certificate of Analysis

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Product Specific Notices for Tubulin alpha-8 Antibody (OTI2G6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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