TumorTACS In Situ Apoptosis Detection Kit

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues
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TumorTACS In Situ Apoptosis Detection Kit_Mammary Tumor_4815-30-K
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Product Details
Citations (20)

TumorTACS In Situ Apoptosis Detection Kit Summary

A complete TUNEL assay based kit tailored for the detection of DNA fragmentation associated with apoptosis in tumors or cancer cells.

Key Benefits

• Unique buffer system produces more consistent labeling.
• Performance tested on tumor samples.
• Includes both Proteinase K and  exclusive non-enzymatic Cytonin permeabilization reagent.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
• Helps resolve unique problems encountered when using tissues or cells from tumors.

Why Use TumorTACS In Situ Apoptosis Detection Kit?

The labeling of tumor-containing specimens can be problematic due to the high levels of necrosis in these tissues. During necrosis, cessation of homeostasis, cell swelling, and nuclear membrane rupture generates highly degraded DNA that can interfere with interpretation of data.

Product Specifications

  • In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Assists in the identification of apoptotic morphologies.

Kit Contents

• Proteinase K
• Streptavidin-HRP
• Diaminobenzidine
• DAB Enhancer
• TACS-Nuclease
• TACS-Nuclease Buffer
• Methyl Green 1%
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TdT Enzyme
• 50x Manganese Cation
• Cytonin


Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.


For research use only. Not for diagnostic use.

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Data Example

Apoptotic Cells within a Mouse Mammary Tumor Identified using the TumorTACS In Situ Apoptosis Detection Kit. Mammary tumor tissue was fixed in 4% paraformaldehyde overnight and paraffin-embedded. Five micron sections were prepared and placed onto glass microscope slides. The sample was processed following the TumorTACS Kit protocol (Catalog # 4815-30-K). Brown stained nuclei indicate apoptotic cells.

Citations for TumorTACS In Situ Apoptosis Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Multi-Lens Arrays (MLA)-Assisted Photothermal Effects for Enhanced Fractional Cancer Treatment: Computational and Experimental Validations
    Authors: H Kim, H Pyo, H Kim, HW Kang
    Cancers, 2021;13(5):.  2021
  2. Co-administration of H-ferritin-doxorubicin and Trastuzumab in neoadjuvant setting improves efficacy and prevents cardiotoxicity in HER2?+?murine breast cancer model
    Authors: F Andreata, A Bonizzi, M Sevieri, M Truffi, M Monieri, L Sitia, F Silva, L Sorrentino, R Allevi, P Zerbi, B Marchini, E Longhi, R Ottria, S Casati, R Vanna, C Morasso, M Bellini, D Prosperi, F Corsi, S Mazzucchel
    Sci Rep, 2020;10(1):11425.  2020
  3. Tussilagone Reduces Tumorigenesis by Diminishing Inflammation in Experimental Colitis-Associated Colon Cancer
    Authors: SH Nam, JK Kim
    Biomedicines, 2020;8(4):.  2020
  4. Inhibition of Heparanase Expression Results in Suppression of Invasion, Migration and Adhesion Abilities of Bladder Cancer Cells
    Authors: Y Tatsumi, M Miyake, K Shimada, T Fujii, S Hori, Y Morizawa, Y Nakai, S Anai, N Tanaka, N Konishi, K Fujimoto
    Int J Mol Sci, 2020;21(11):.  2020
  5. SPARC correlates with unfavorable outcome and promotes tumor growth in lung squamous cell carcinoma
    Authors: J Xu, S Yang, X Gu, H Shen, L Wang, W Xu, L Fang, Y Mao, L Xu, Y Chen, W Fan, J Wang
    Exp. Mol. Pathol., 2019;0(0):104276.  2019
  6. Non-obstructive vas deferens and epididymis loss in cystic fibrosis rats
    Authors: ZE Plyler, SE Birket, BD Schultz, JS Hong, SM Rowe, CF Petty, MR Crowley, DK Crossman, TR Schoeb, EJ Sorscher
    Mech. Dev., 2019;155(0):15-26.  2019
  7. Combined Treatment with MEK and mTOR Inhibitors is Effective in In Vitro and In Vivo Models of Hepatocellular Carcinoma
    Authors: X Liu, J Hu, X Song, K Utpatel, Y Zhang, P Wang, X Lu, J Zhang, M Xu, T Su, L Che, J Wang, M Evert, DF Calvisi, X Chen
    Cancers (Basel), 2019;11(7):.  2019
  8. IPMK Mediates Activation of ULK Signaling and Transcriptional Regulation of Autophagy Linked to Liver Inflammation and Regeneration
    Authors: P Guha, R Tyagi, S Chowdhury, L Reilly, C Fu, R Xu, AC Resnick, SH Snyder
    Cell Rep, 2019;26(10):2692-2703.e7.  2019
  9. Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression
    Authors: SP Kalainayak, P Ghosh, S Dey, KE Fitzgerald, S Sohoni, PC Konduri, M Garrossian, L Liu, L Zhang
    Sci Rep, 2019;9(1):1405.  2019
  10. Mucin 1 is a potential therapeutic target in cutaneous T-cell lymphoma.
    Authors: Jain S, Stroopinsky D, Yin L, Rosenblatt J, Alam M, Bhargava P, Clark R, Kupper T, Palmer K, Coll M, Rajabi H, Pyzer A, Bar-Natan M, Luptakova K, Arnason J, Joyce R, Kufe D, Avigan D
    Blood, 2015;126(3):354-62.  2015
  11. Preclinical pharmacologic evaluation of pralatrexate and romidepsin confirms potent synergy of the combination in a murine model of human T-cell lymphoma.
    Authors: Jain S, Jirau-Serrano X, Zullo K, Scotto L, Palermo C, Sastra S, Olive K, Cremers S, Thomas T, Wei Y, Zhang Y, Bhagat G, Amengual J, Deng C, Karan C, Realubit R, Bates S, O'Connor O
    Clin Cancer Res, 2015;21(9):2096-106.  2015
  12. Nitidine chloride inhibits hepatic cancer growth via modulation of multiple signaling pathways.
    Authors: Lin, Jiumao, Shen, Aling, Chen, Hongwei, Liao, Jun, Xu, Teng, Liu, Liya, Lin, Jing, Peng, Jun
    BMC Cancer, 2014;14(0):729.  2014
  13. TNF-related apoptosis-inducing ligand enhances vinorelbine-induced apoptosis and antitumor activity in a preclinical model of non-small cell lung cancer.
    Authors: Zhu K, Fang W, Chen Y, Lin S, Chen X
    Oncol Rep, 2014;32(3):1234-42.  2014
  14. Distinct phospholipase C-beta isozymes mediate lysophosphatidic acid receptor 1 effects on intestinal epithelial homeostasis and wound closure.
    Authors: Lee S, Leoni G, Neumann P, Chun J, Nusrat A, Yun C
    Mol Cell Biol, 2013;33(10):2016-28.  2013
  15. Recombinant VP1, an Akt inhibitor, suppresses progression of hepatocellular carcinoma by inducing apoptosis and modulation of CCL2 production.
    Authors: Chen TA, Wang JL, Hung SW, Chu CL, Cheng YC, Liang SM
    PLoS ONE, 2011;6(8):e23317.  2011
  16. Molecular chaperone gp96 is a novel therapeutic target of multiple myeloma.
    Authors: Hua Y, White-Gilbertson S, Kellner J, Rachidi S, Usmani S, Chiosis G, Depinho R, Li Z, Liu B
    Clin Cancer Res, 0;19(22):6242-51.  0
  17. Rapamycin induces Bad phosphorylation in association with its resistance to human lung cancer cells.
    Authors: Liu Y, Sun S, Owonikoko T, Sica G, Curran W, Khuri F, Deng X
    Mol Cancer Ther, 0;11(1):45-56.  0
  18. Anticancer drug candidate CBL0137, which inhibits histone chaperone FACT, is efficacious in preclinical orthotopic models of temozolomide-responsive and -resistant glioblastoma.
    Authors: Barone T, Burkhart C, Safina A, Haderski G, Gurova K, Purmal A, Gudkov A, Plunkett R
    Neuro Oncol, 0;19(2):186-196.  0
  19. Experimental mild renal insufficiency mediates early cardiac apoptosis, fibrosis, and diastolic dysfunction: a kidney-heart connection.
    Authors: Martin F, McKie P, Cataliotti A, Sangaralingham S, Korinek J, Huntley B, Oehler E, Harders G, Ichiki T, Mangiafico S, Nath K, Redfield M, Chen H, Burnett J
    Am J Physiol Regul Integr Comp Physiol, 0;302(2):R292-9.  0
  20. Bifurcation of axons from cranial sensory neurons is disabled in the absence of Npr2-induced cGMP signaling.
    Authors: Ter-Avetisyan G, Rathjen F, Schmidt H
    J Neurosci, 0;34(3):737-47.  0


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