TumorTACS In Situ Apoptosis Detection Kit

Name: TumorTACS In Situ Apoptosis Detection Kit
Brand: R&D Systems
SKU: 4815-30-K

R&D Systems | Catalog # 4815-30-K

Submit a review

Citations (24)

Product Documents

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Discontinued Product

4815-30-K has been discontinued. View all TUNEL Assays products.

Product Summary
Scientific Data
Kit Contents
Preparation & Storage
Product Documents

Key Product Details

Features

A complete TUNEL assay based kit tailored for the detection of DNA fragmentation associated with apoptosis in tumors or cancer cells.

Key Benefits

Unique buffer system produces more consistent labeling.
Performance tested on tumor samples.
Includes both Proteinase K and exclusive non-enzymatic Cytonin permeabilization reagent.
Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
Helps resolve unique problems encountered when using tissues or cells from tumors.

Product Summary for TumorTACS In Situ Apoptosis Detection Kit

Why Use TumorTACS In Situ Apoptosis Detection Kit?

The labeling of tumor-containing specimens can be problematic due to the high levels of necrosis in these tissues. During necrosis, cessation of homeostasis, cell swelling, and nuclear membrane rupture generates highly degraded DNA that can interfere with interpretation of data.

Product Specifications

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
Assists in the identification of apoptotic morphologies.

Scientific Data Images for TumorTACS In Situ Apoptosis Detection Kit

Apoptotic Cells within a Mouse Mammary Tumor Identified using the TumorTACS In Situ Apoptosis Detection Kit.

Mammary tumor tissue was fixed in 4% paraformaldehyde overnight and paraffin-embedded. Five micron sections were prepared and placed onto glass microscope slides. The sample was processed following the TumorTACS Kit protocol (Catalog # 4815-30-K). Brown stained nuclei indicate apoptotic cells.

Kit Contents for TumorTACS In Situ Apoptosis Detection Kit

Proteinase K
Streptavidin-HRP
Diaminobenzidine
DAB Enhancer
TACS-Nuclease
TACS-Nuclease Buffer
Methyl Green 1%
TACS 2 TdT Labeling Buffer
TACS 2 TdT Stop Buffer
TACS 2 TdT dNTP
TdT Enzyme
50x Manganese Cation
Cytonin

Formulation, Preparation, and Storage

Shipping

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Product Documents for TumorTACS In Situ Apoptosis Detection Kit

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Citations for TumorTACS In Situ Apoptosis Detection Kit

Customer Reviews for TumorTACS In Situ Apoptosis Detection Kit

There are currently no reviews for this product. Be the first to review TumorTACS In Situ Apoptosis Detection Kit and earn rewards!

Have you used TumorTACS In Situ Apoptosis Detection Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image