TumorTACS In Situ Apoptosis Detection Kit

TumorTACS In Situ Apoptosis Detection Kit Summary

Key Benefits

• Unique buffer system produces more consistent labeling.
• Performance tested on tumor samples.
• Includes both Proteinase K and  exclusive non-enzymatic Cytonin permeabilization reagent.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
• Helps resolve unique problems encountered when using tissues or cells from tumors.

Why Use TumorTACS In Situ Apoptosis Detection Kit?

The labeling of tumor-containing specimens can be problematic due to the high levels of necrosis in these tissues. During necrosis, cessation of homeostasis, cell swelling, and nuclear membrane rupture generates highly degraded DNA that can interfere with interpretation of data.

Product Specifications

• In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
• Assists in the identification of apoptotic morphologies.

Kit Contents

• Proteinase K
• Streptavidin-HRP
• Diaminobenzidine
• DAB Enhancer
• TACS-Nuclease
• TACS-Nuclease Buffer
• Methyl Green 1%
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Manganese Cation
• Cytonin

Specifications

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

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Apoptotic Cells within a Mouse Mammary Tumor Identified using the TumorTACS In Situ Apoptosis Detection Kit. Mammary tumor tissue was fixed in 4% paraformaldehyde overnight and paraffin-embedded. Five micron sections were prepared and placed onto glass microscope slides. The sample was processed following the TumorTACS Kit protocol (Catalog # 4815-30-K). Brown stained nuclei indicate apoptotic cells.