Twist-1 Antibody (10E4E6) - BSA Free

Novus Biologicals | Catalog # NBP2-37364

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Knockdown Validated, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10E4E6

Format

BSA Free
View all Twist-1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Twist-1 (AA: 9-74) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Twist-1 Antibody (10E4E6) - BSA Free

Western Blot: Twist-1 Antibody (10E4E6)BSA Free [NBP2-37364]

Western Blot: Twist-1 Antibody (10E4E6)BSA Free [NBP2-37364]

Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Immunocytochemistry/ Immunofluorescence: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Immunocytochemistry/Immunofluorescence: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of HeLa cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - IHC staining of paraffin-embedded squamous cell carcinoma sample using Twist-1 antibody at 1:100 dilution. IHC-P image submitted by a verified customer review.
Flow Cytometry: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Flow Cytometry: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on SH-SY5Y cells with Twist-1 Antibody (10E4E6) NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.
Western Blot: Twist-1 Antibody (10E4E6)BSA Free [NBP2-37364]

Western Blot: Twist-1 Antibody (10E4E6)BSA Free [NBP2-37364]

Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mAb against human TWIST1 recombinant protein. Expected MW is 31.9 kDa.
Western Blot: Twist-1 Antibody (10E4E6)BSA Free [NBP2-37364]

Western Blot: Twist-1 Antibody (10E4E6)BSA Free [NBP2-37364]

Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Western blot analysis using TWIST1 mAb against HEK293 (1) and TWIST1 (AA: 9-74)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of paraffin-embedded cervical cancer tissues using TWIST1 mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.
Flow Cytometry: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Flow Cytometry: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).
Flow Cytometry: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Flow Cytometry: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.
Flow (Intracellular): Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Flow (Intracellular): Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

Flow (Intracellular): Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image from the DyLight 550 version of this antibody.
ELISA: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

ELISA: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364]

ELISA: Twist-1 Antibody (10E4E6) [NBP2-37364] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Twist-1 Antibody (10E4E6) - BSA Free

Western Blot: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364] -

Western Blot: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364] - Validation of novel candidates at cellular DNA replication forks.BL cells (A) & LCL (B) cells were labeled with EdU for 15 min (active) followed by exposure to HU for 2 hours (stalled) prior to performing iPOND. Samples isolated by iPOND & 0.1% input samples were subjected to immunoblotting with indicated antibodies. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31841561), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Twist-1 Antibody (10E4E6) - BSA Free

Western Blot: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364] -

Western Blot: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364] - Validation of novel candidates at cellular DNA replication forks.BL cells (A) & LCL (B) cells were labeled with EdU for 15 min (active) followed by exposure to HU for 2 hours (stalled) prior to performing iPOND. Samples isolated by iPOND & 0.1% input samples were subjected to immunoblotting with indicated antibodies. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31841561), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Twist-1 Antibody (10E4E6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is CyTOF ready. Use in Knockdown Validated reported in scientific literature (PMID: 31113881).

Reviewed Applications

Read 1 review rated 5 using NBP2-37364 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Twist-1

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq]

Long Name

Twist Related Protein 1 [Drosophila]

Alternate Names

ACS3, BPES3, H-twist, Twist1

Gene Symbol

TWIST1

Additional Twist-1 Products

Product Documents for Twist-1 Antibody (10E4E6) - BSA Free

Certificate of Analysis

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Product Specific Notices for Twist-1 Antibody (10E4E6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Twist-1 Antibody (10E4E6) - BSA Free

Customer Reviews for Twist-1 Antibody (10E4E6) - BSA Free (1)

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  • Twist-1 Antibody (10E4E6)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Squamous cell carcinoma
    Species: Human
    Verified Customer | Posted 09/27/2021
    IHC staining of paraffin-embedded squamous cell carcinoma sample using Twist-1 antibody 1:100 dilution
    Twist-1 Antibody (10E4E6) - BSA Free NBP2-37364

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