TXNDC5 Antibody (2E7/7) - BSA Free

Novus Biologicals | Catalog # NBP2-22414

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 2E7/7

Format

BSA Free
View all TXNDC5 Primary Antibodies »

Product Specifications

Immunogen

Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein

Localization

Endoplasmic Reticulum, Endoplasmic Reticulum Lumen

Specificity

Detects approx 48kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TXNDC5 Antibody (2E7/7) - BSA Free

Immunocytochemistry/ Immunofluorescence: TXNDC5 Antibody (2E7/7) [NBP2-22414]

Immunocytochemistry/ Immunofluorescence: TXNDC5 Antibody (2E7/7) [NBP2-22414]

Immunocytochemistry/Immunofluorescence: TXNDC5 Antibody (2E7/7) [NBP2-22414] - Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EndoPDI Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.

Applications for TXNDC5 Antibody (2E7/7) - BSA Free

Application
Recommended Usage

Western Blot

1:200
Application Notes
1 ug/ml of EndoPDI Antibody was sufficient for detection of EndoPDI in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TXNDC5

TXNDC5 encodes a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. This gene can be co-transcribed with the upstream gene MU.

Long Name

Thioredoxin Domain Containing 5

Alternate Names

EndoPDI, ERp46, TLP46

Gene Symbol

TXNDC5

UniProt

Q8NBS9 (Human)

Additional TXNDC5 Products

Product Documents for TXNDC5 Antibody (2E7/7) - BSA Free

Certificate of Analysis

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Product Specific Notices for TXNDC5 Antibody (2E7/7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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