TXNDC5 Antibody

Novus Biologicals | Catalog # NB100-1452

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TXNDC5 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SLHRFVLSQAKDEL corresponding to C-Terminus according to NP_110437.2, NP_001139021.1.

Specificity

This antibody is expected to be able to recognize both reported human isoforms, as represented by NP_110437.2; NP_001139021.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TXNDC5 Antibody

Western Blot: TXNDC5 Antibody [NB100-1452]

Western Blot: TXNDC5 Antibody [NB100-1452]

Western Blot: TXNDC5 Antibody [NB100-1452] - Staining of HEK293 (A), A549 (B), HeLa (C) with antibody at 0.1 ug/mL and of HepG2 (D) cell lysate with antibody at 0.01 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: TXNDC5 Antibody [NB100-1452]

Immunocytochemistry/ Immunofluorescence: TXNDC5 Antibody [NB100-1452]

Immunocytochemistry/Immunofluorescence: TXNDC5 Antibody [NB100-1452] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: TXNDC5 Antibody [NB100-1452]

Immunohistochemistry-Paraffin: TXNDC5 Antibody [NB100-1452]

Immunohistochemistry-Paraffin: TXNDC5 Antibody [NB100-1452] - Staining of paraffin embedded Human Small Intestine. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: TXNDC5 Antibody [NB100-1452]

Flow Cytometry: TXNDC5 Antibody [NB100-1452]

Flow Cytometry: TXNDC5 Antibody [NB100-1452] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: TXNDC5 Antibody [NB100-1452]

Immunocytochemistry/ Immunofluorescence: TXNDC5 Antibody [NB100-1452]

Immunocytochemistry/Immunofluorescence: TXNDC5 Antibody [NB100-1452] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for TXNDC5 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

3.75 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.01 - 0.1 ug/mL
Application Notes
Western blot: Approx 50 kDa band observed in lysates of cell lines HEK293, A549, HeLa and HepG2 (calculated MW of 47.6 kDa according to NP_110437.2).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TXNDC5

This is a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis.

Long Name

Thioredoxin Domain Containing 5

Alternate Names

EndoPDI, ERp46, TLP46

Entrez Gene IDs

81567 (Human)

Gene Symbol

TXNDC5

UniProt

NP_110437 (Human)

Additional TXNDC5 Products

Product Documents for TXNDC5 Antibody

Certificate of Analysis

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Product Specific Notices for TXNDC5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TXNDC5 Antibody

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Protocols

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