TXNIP Antibody (JY2) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-81001
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # JY2
Format
Azide and BSA Free
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Product Specifications
Immunogen
Human recombinant TXNIP
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TXNIP Antibody (JY2) - Azide and BSA Free
![Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP2-81001]](https://resources.rndsystems.com/images/products/TXNIP-Antibody-JY2-Azide-and-BSA-Free-Western-Blot-NBP2-81001-img0002.jpg "Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP2-81001]")
Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP2-81001]
Western Blot: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001] - TXNIP expression in Raji (1), K562 (2), KG1 (3), MRC5 (4), IC2Tr (5), HEL (6), P19 (7) and WR19L (8). Image from the standard format of this antibody.![Immunocytochemistry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]](https://resources.rndsystems.com/images/products/TXNIP-Antibody-JY2-Azide-and-BSA-Free-Immunocytochemistry-NBP2-81001-img0005.jpg "Immunocytochemistry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]")
Immunocytochemistry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]
Immunocytochemistry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001] - Formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:250 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent which followed counterstaining of the cell nuclei with![Immunohistochemistry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]](https://resources.rndsystems.com/images/products/TXNIP-Antibody-JY2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-81001-img0004.jpg "Immunohistochemistry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]")
Immunohistochemistry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]
Immunohistochemistry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001] - Formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:100 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent which followed counterstaining of the cell nuclei with![Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]](https://resources.rndsystems.com/images/products/TXNIP-Antibody-JY2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-81001-img0006.jpg "Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]")
Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]
Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001] - An intracellular stain was performed on RH-30 cells with TXNIP (JY2) Antibody NBP1-54578F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.![Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP2-81001]](https://resources.rndsystems.com/images/products/TXNIP-Antibody-JY2-Azide-and-BSA-Free-Western-Blot-NBP2-81001-img0001.jpg "Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP2-81001]")
Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP2-81001]
Western Blot: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001] - Hepa1 lysates probed with anti-TXNIP (JY2) at 1:2K. Cells were transfected with either empty vector or mouse TXNIP expression vector (MYC/DDK tagged). 30ug protein per lane. Image from verified customer review. Image from the standard format of this antibody.![Immunohistochemistry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]](https://resources.rndsystems.com/images/products/TXNIP-Antibody-JY2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-81001-img0003.jpg "Immunohistochemistry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]")
Immunohistochemistry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]
Immunohistochemistry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001] - TXNIP in mouse brain. Antibody dilution: 1:100, incubated overnight in 5%BSA + 0.2% Triton X 100. Image from verified customer review. Image from the standard format of this antibody.![Immunoprecipitation: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]](https://resources.rndsystems.com/images/products/TXNIP-Antibody-JY2-Azide-and-BSA-Free-Immunoprecipitation-NBP2-81001-img0007.jpg "Immunoprecipitation: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]")
Immunoprecipitation: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001]
Immunoprecipitation: TXNIP Antibody (JY2) - Azide and BSA Free [NBP2-81001] - Txnip/VDUP1 from Raji with NBP1-54578 (1) or mouse IgG1 (2). Image from the standard format of this antibody.Applications for TXNIP Antibody (JY2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
2-5 ug/0.1x10^6 cells
Immunohistochemistry-Frozen
reported by customer review
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
5x10^6 cells
Western Blot
1:1000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TXNIP
Alternate Names
HHCPA78, THIF, thioredoxin binding protein 2, thioredoxin interacting protein, Thioredoxin-binding protein 2, thioredoxin-interacting protein, upregulated by 1,25-dihydroxyvitamin D-3, VDUP1EST01027, Vitamin D3 up-regulated protein 1
Gene Symbol
TXNIP
Additional TXNIP Products
Product Documents for TXNIP Antibody (JY2) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TXNIP Antibody (JY2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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