TXNIP Antibody (JY2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-54578

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Amphibian

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Simple Western, Immunoprecipitation, Proximity Ligation Assay, CyTOF-ready

Cited:

Knockout Validated, Immunohistochemistry-Paraffin, Western Blot, Simple Western, Immunoprecipitation, Proximity Ligation Assay, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # JY2

Format

Azide and BSA Free
View all TXNIP Primary Antibodies »

Product Specifications

Immunogen

Human recombinant TXNIP

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34576095). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TXNIP Antibody (JY2) - Azide and BSA Free

Immunohistochemistry-Frozen: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunohistochemistry-Frozen: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunohistochemistry-Frozen: TXNIP Antibody (JY2) [NBP1-54578] - TXNIP in mouse brain. Antibody dilution: 1:100, incubated overnight in 5%BSA + 0.2% Triton X 100. Image from verified customer review.
Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - TXNIP expression in Raji (1), K562 (2), KG1 (3), MRC5 (4), IC2Tr (5), HEL (6), P19 (7) and WR19L (8).
Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

TXNIP-Antibody-JY2-Azide-and-BSA-Free-Western-Blot-NBP1-54578-img0011.jpg
Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578] - An intracellular stain was performed on THP-1 cells with TXNIP Antibody (JY2) NBP1-54578AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - Hepa1 lysates probed with anti-TXNIP (JY2) at 1:2K. Cells were transfected with either empty vector or mouse TXNIP expression vector (MYC/DDK tagged). 30ug protein per lane. Image from verified customer review.
Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2)Azide and BSA Free [NBP1-54578]

Western Blot: TXNIP Antibody (JY2) [NBP1-54578] - AMPK mediates anti-pyroptotic effects of Exendin-4. (a) ROS determination by FACS. The left arrow indicated the ROS-negative population and the right pointed the positive. (b) Transcription activity of TXNIP in cardiomyocytes. (c) Western blot. (d) RNA silencing of TXNIP in cardiomyocytes. (e) IL-1 ELISA with TXNIP RNAi. (f) Caspase-1 activity assay with TXNIP RNAi. (g) Quantification of pAMPK/AMPK with CC treatment. (h) IL-1 ELISA with CC treatment. (i) Caspase-1 activity assay with CC treatment. Values are the mean SEM of 3 samples per group.0.05,0.01,0.005, and0.001. Exendin-4 Protects against Hyperglycemia-Induced Cardiomyocyte Pyroptosis via the AMPK-TXNIP Pathway. J Diabetes Res (2019)
Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) [NBP1-54578] - Formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:250 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent which followed counterstaining of the cell nuclei with hematoxylin. This TXNIP antibody generated a diffused cytoplasmic signal in the cells of various tubules and glomeruli.
Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunohistochemistry-Paraffin: TXNIP Antibody (JY2) [NBP1-54578] - Formalin fixed and paraffin embedded tissue section of mouse kidney using TXNIP antibody (clone JY2) at 1:100 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent which followed counterstaining of the cell nuclei with hematoxylin. This TXNIP antibody generated a diffused cytoplasmic signal in the cells of various tubules with negligible staining in glomeruli.
Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Flow Cytometry: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Flow Cytometry: TXNIP Antibody (JY2) [NBP1-54578] - An intracellular stain was performed on RH-30 cells with TXNIP (JY2) Antibody NBP1-54578F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.
Immunoprecipitation: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunoprecipitation: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578]

Immunoprecipitation: TXNIP Antibody (JY2) [NBP1-54578] - Txnip/VDUP1 from Raji with NBP1-54578 (1) or mouse IgG1 (2).
TXNIP Antibody (JY2) - Azide and BSA Free

Western Blot: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578] -

Western Blot: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578] - AMPK mediates anti-pyroptotic effects of Exendin-4. (a) ROS determination by FACS. The left arrow indicated the ROS-negative population & the right pointed the positive. (b) Transcription activity of TXNIP in cardiomyocytes. (c) Western blot. (d) RNA silencing of TXNIP in cardiomyocytes. (e) IL-1 beta ELISA with TXNIP RNAi. (f) Caspase-1 activity assay with TXNIP RNAi. (g) Quantification of pAMPK/AMPK with CC treatment. (h) IL-1 beta ELISA with CC treatment. (i) Caspase-1 activity assay with CC treatment. Values are the mean ± SEM of 3 samples per group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005, & ∗∗∗∗p < 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31886288), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TXNIP Antibody (JY2) - Azide and BSA Free

Simple Western: TXNIP Antibody (JY2) - Azide and BSA Free [NBP1-54578] -

Proinflammatory cytokine (IL-6) and TXNIP expression and TUNEL assay for STZ-induced mice treated with Verapamil (V) and R-Vera (RV).(A) STZ-induced mice administered 100 mg/kg/day racemic or R-Vera exhibited significantly reduced serum IL-6 expression compared to vehicle mice whereas those administered 50 mg/kg/day R-Vera did not. **P < 0.01 indicates significant difference from vehicle using one-way ANOVA. N = 8 in each group. (B) STZ-induced mice showed highly upregulated islet tissue TXNIP compared to normal C57BL/6J mice. TXNIP was downregulated after racemic or R-Vera administration compared to vehicle. #P < 0.05 indicated significant difference between normal and vehicle; *P < 0.05 indicated significant difference to vehicle using one-way ANOVA. N = 8 in each group. (C) beta -cell apoptosis assessed using TUNEL staining. Deep staining spots in images are apoptotic beta -cells (200×). (D) TUNEL-positive cell counts show that STZ-induced mice administered 100 mg/kg/day R-Vera had significantly reduce beta -cell apoptosis. *P < 0.05 indicated a significant difference between vehicle and RV-100 using one-way ANOVA. The sample numbers of 5, 7, 5, and 9 indicate the section numbers from the Vehicle, V-100, RV-100, and RV-50 groups, respectively (rather than the number of mice). Each data point represents the percentage of TUNEL–positive cells per slide in each group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34358247), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TXNIP Antibody (JY2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

2-5 ug/0.1x10^6 cells

Immunohistochemistry-Frozen

reported by customer review

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2ug/200ul of cell extract from 5x10^6 cells

Knockout Validated

reported in scientific literature (Brocker et al)

Simple Western

1:500

Western Blot

1 ug/ml

Reviewed Applications

Read 3 reviews rated 4.3 using NBP1-54578 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TXNIP

Vitamin D3 up-regulated protein 1 (VDUP-1), also called Thioredoxin (TRX)-interacting protein, is an endogenous inhibitor of TRX. Redox-dependent regulation of VDUP-1 by mitogenic factors through Reactive oxygen species (ROS) and the specific binding of VDUP-1 to the redox-sensitive cysteine-sulfide center of TRX modulate intracellular levels of ROS and the mitogenic activity of TRX. It has been reported that TXNIP plays important roles in diverse cellular processes, including the regulation of cellular redox balance, apoptosis, proliferation, and differentiation.

Alternate Names

HHCPA78, THIF, thioredoxin binding protein 2, thioredoxin interacting protein, Thioredoxin-binding protein 2, thioredoxin-interacting protein, upregulated by 1,25-dihydroxyvitamin D-3, VDUP1EST01027, Vitamin D3 up-regulated protein 1

Entrez Gene IDs

10628 (Human); 56338 (Mouse); 117514 (Rat)

Gene Symbol

TXNIP

UniProt

Q9H3M7 (Human)

Additional TXNIP Products

Product Documents for TXNIP Antibody (JY2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TXNIP Antibody (JY2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TXNIP Antibody (JY2) - Azide and BSA Free

Customer Reviews for TXNIP Antibody (JY2) - Azide and BSA Free (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used TXNIP Antibody (JY2) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 of 3 reviews Showing All
Filter By:
Clear Filters X
  • TXNIP Antibody (JY2)
    Name: Saifudeen Ismael
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 02/28/2019
    TXNIP in mouse brain
    Antibody dilution: 1:100, incubated overnight in 5%BSA + 0.2% Triton X 100.
    TXNIP Antibody (JY2) - Azide and BSA Free NBP1-54578
  • TXNIP Antibody (JY2)
    Name: Saifudeen Ismael
    Application: Western Blot
    Sample Tested: Mouse brain cortex whole lysate and Mouse brain
    Species: mouse brain and Mouse
    Verified Customer | Posted 08/22/2018
    TXNIP expression in mouse brain
    TXNIP Antibody (JY2) - Azide and BSA Free NBP1-54578
  • TXNIP Antibody (JY2)
    Name: Chad Brocker
    Application: Western Blot
    Sample Tested: Hepa1 cells transfected with mouse TXNIP expression vector and Hepa1 whole cell lysates
    Species: Mouse
    Verified Customer | Posted 05/22/2018
    Hepa1 lysates probed with anti-TXNIP (JY2) @ 1:2K. Cells were transfected with either empty vector or mouse TXNIP expression vector (MYC/DDK tagged).
    Hepa1 lysates probed with anti-TXNIP (JY2) @ 1:2K. Cells were transfected with either empty vector or mouse TXNIP expression vector (MYC/DDK tagged). Loaded 30ug protein per lane.
    TXNIP Antibody (JY2) - Azide and BSA Free NBP1-54578

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies