Tyrosine Hydroxylase [p Ser31] Antibody - Azide Free

Novus Biologicals | Catalog # NB300-211

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Tyrosine Hydroxylase Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyrosine Hydroxylase conjugated to KLH. Accession # P04177

Reactivity Notes

Use in Human reported in scientific literature (PMID:32796843).

Modification

p Ser31

Localization

Cytoplasmic

Marker

Neuronal Marker

Specificity

Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein phosphorylated at Ser31.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Tyrosine Hydroxylase [p Ser31] Antibody - Azide Free (NB300-211) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Tyrosine Hydroxylase Antibody: Cited in 8 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Tyrosine Hydroxylase [p Ser31] Antibody - Azide Free

Western Blot: Tyrosine Hydroxylase [p Ser31] Antibody [NB300-211]

Western Blot: Tyrosine Hydroxylase [p Ser31] Antibody [NB300-211]

Western Blot: Tyrosine Hydroxylase [p Ser31] Antibody [NB300-211] - PC-12 cells incubated in the absence (Control) and presence of okadaic acid (OA, 1 uM for 60 min) showing specific immunolabeling of the ~60 kDa TH phosphorylated at Ser31.

Applications for Tyrosine Hydroxylase [p Ser31] Antibody - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:1000

Immunohistochemistry-Frozen

1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tyrosine Hydroxylase

The tetrameric enzyme tyrosine hydroxylase (TH), also designated as tyrosine 3-monooxygenase (TY3H), is the rate-limiting enzyme for catecholamine biosynthesis and has a theoretical molecular weight of 60 kDa. Encoded by the TH gene, TH catalyzes the conversion of the amino acid L-tyrosine to L-3,4- dihydroxyphenylalanine (L-dopa) in the central nervous system and adrenal medulla. The precursor of catecholamines, L-dopa, is converted to dopamine for use in the biosynthesis of neurotransmitters norepinephrine (noradrenaline) and epinephrine (adrenaline) (1). Dysfunction in the TH gene, and thus the enzymatic activity by TH, dysregulates catecholamine synthesis.

Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. Dysregulation of breakdown for the amino acid, tyrosine, by TH is a result of a genetic disorder that results in Tyrosinemia (high levels of tyrosine in the blood, tissue and organs).

Tyrosine hydroxylase deficiency is a disorder that primarily affects movement, where individuals display symptoms that include lack of coordination when walking, postural tremors and unusual body positioning. TH deficient dopamine-responsive dystonia (DRD), also known as Segawa syndrome, is a rare genetic disorder that is associated with low levels of TH and is diagnosed during childhood with characteristic symptoms including increased muscle tone (dystonia) and signs of Parkinsonism like bradykinesia, tremors, rigidity and postural instability (2). Correspondingly, TH is also linked to Parkinson's disease in older adults, where low dopamine levels are a consistent neurochemical abnormality. Functional polymorphisms of the TH gene may be involved in the pathogenesis of neuropsychiatric diseases such as schizophrenia and other affective disorders where dopamine is often dysregulated (3).

References

1. Hamanaka, Y., & Mizunami, M. (2019). Tyrosine hydroxylase-immunoreactive neurons in the mushroom body of the field cricket, Gryllus bimaculatus. Cell Tissue Res, 376(1), 97-111. doi:10.1007/s00441-018-2969-9

2. Li, L., & Zhou, F. M. (2013). Parallel dopamine D1 receptor activity dependence of l-Dopa-induced normal movement and dyskinesia in mice. Neuroscience, 236, 66-76. doi:10.1016/j.neuroscience.2012.12.065

3. Borkar, C. D., Bharne, A. P., Nagalakshmi, B., Sakharkar, A. J., Subhedar, N. K., & Kokare, D. M. (2018). Cocaine- and Amphetamine-Regulated Transcript Peptide (CART) Alleviates MK-801-Induced Schizophrenic Dementia-Like Symptoms. Neuroscience, 375, 94-107. doi:10.1016/j.neuroscience.2018.01.056

Alternate Names

TH, TYH

Entrez Gene IDs

21823 (Mouse); 25085 (Rat)

Gene Symbol

TH

UniProt

P04177 (Rat)

Additional Tyrosine Hydroxylase Products

Product Documents for Tyrosine Hydroxylase [p Ser31] Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Tyrosine Hydroxylase [p Ser31] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Tyrosine Hydroxylase [p Ser31] Antibody - Azide Free

Customer Reviews for Tyrosine Hydroxylase [p Ser31] Antibody - Azide Free

There are currently no reviews for this product. Be the first to review Tyrosine Hydroxylase [p Ser31] Antibody - Azide Free and earn rewards!

Have you used Tyrosine Hydroxylase [p Ser31] Antibody - Azide Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies