U0126

Tocris Bioscience | Catalog # 1144

Potent, selective inhibitor of MEK1 and 2
Tocris Bioscience

Key Product Details

Description

Potent, selective inhibitor of MEK1 and 2
View all MEK Inhibitors »

Product Description

U0126 is a potent and selective non-competitive inhibitor of MAP kinase kinase. Inhibits MEK-1 and MEK-2 (IC50 values of 0.07 and 0.06 μM respectively) with little or no effect on the activities of PKC, Abl, Raf, MEKK, ERK, JNK, MKK-3, MKK-4/SEK, MKK-6, Cdk2 or Cdk4. Brain penetrant. Helps maintain human pluripotent stem cells in undifferentiated state.

Negative Control also available.

Product Specifications for U0126

Molecular Weight

380.48

Formula

C18H16N6S2

Storage

Desiccate at +4°C

Chemical Name

1,4-Diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene

CAS Number

109511-58-2

PubChem ID

3006531

InChI Key

DVEXZJFMOKTQEZ-JYFOCSDGSA-N

SMILES

N\C(SC1=CC=CC=C1N)=C(C#N)/C(C#N)=C(\N)SC1=CC=CC=C1N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 38.05 100

Preparing Stock Solutions for U0126

The following data is based on the product molecular weight 380.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.63 mL 13.14 mL 26.28 mL
5 mM 0.53 mL 2.63 mL 5.26 mL
10 mM 0.26 mL 1.31 mL 2.63 mL
50 mM 0.05 mL 0.26 mL 0.53 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 680 publications citing the usage of this product.

Product Documents for U0126

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for U0126

For research use only

Customer Reviews for U0126 (5)

4.8 out of 5
5 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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1 Stars
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Showing  1 - 5 of 5 reviews Showing All
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  • Experiment
    Name: Chi Ngo
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Gastic epithelical cells (AGS)
    Verified Customer | Posted 09/28/2024
    Aliquot the reconstituted compound to avoid freezing and thawing repeatedly.
    To investigate if the induction of Snail1 by Helicobacter pylori infection in gastric epithelial cells AGS was regulated by the MEK/ERK pathway.
    U0126 1144
  • Used to block MEK signaling.
    Name: MARINA KOUTSIOUMPA
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 10/13/2022
    Pancreatic cancer cells were pretreated for 2 hours with U0126 followed by conventional chemotherapeutics. MEK inhibition had an additive effect on cancer cell’s viability upon 3 days of treatment.
  • Used for Migration assays
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: A549 or MDAMB231
    Verified Customer | Posted 07/01/2020
    We ordered this product, combining with another inhibitor, to confirm the migration.To confirm that tested product induces migration, we also screen all MAPK inhibitors and MIF receptor antagonists
    All MAPK inhibitors (ZM336372: c-RAF inhibitor; UO126: MEK1/MEK2 inhibitor, SB203580: p38 inhibitor) were used at 10 μM
    U0126 1144
  • Effect of MEK inhibitor on E0771 extracellular flux
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: E0771
    Verified Customer | Posted 10/10/2018
    Extracellular flux analysis (Seahorse experiment) to examine the effect of MEK inhibitor in breast cancer cells. 5-10uM dose used.
  • Fast delivery and great quality
    Name: Anonymous
    Assay Type: In Vitro
    Cell Line/Tissue: CHO-KK1
    Verified Customer | Posted 07/17/2017
    To block the gpcr signaling and determine the pathway of an orphan receptor

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