UAP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32823

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human UAP1. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UAP1 Antibody - BSA Free

Western Blot: UAP1 Antibody [NBP1-32823]

Western Blot: UAP1 Antibody [NBP1-32823]

Western Blot: UAP1 Antibody [NBP1-32823] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:3000
Immunocytochemistry/ Immunofluorescence: UAP1 Antibody [NBP1-32823]

Immunocytochemistry/ Immunofluorescence: UAP1 Antibody [NBP1-32823]

Immunocytochemistry/Immunofluorescence: UAP1 Antibody [NBP1-32823] - UAP1 Antibody detects UAP1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: UAP1 protein stained by UAP1 Antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: UAP1 Antibody [NBP1-32823]

Immunohistochemistry-Paraffin: UAP1 Antibody [NBP1-32823]

Immunohistochemistry-Paraffin: UAP1 Antibody [NBP1-32823] - Paraffin-embedded ES-2 xenograft, using antibody at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: UAP1 Antibody [NBP1-32823]

Immunocytochemistry/ Immunofluorescence: UAP1 Antibody [NBP1-32823]

Immunocytochemistry/Immunofluorescence: UAP1 Antibody [NBP1-32823] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

Applications for UAP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UAP1

Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P

Alternate Names

AgX, AGX1, AGX2, Antigen X, EC 2.7.7, EC 2.7.7.23, EC 2.7.7.27, SPAG2, sperm associated antigen 2, Sperm-associated antigen 2, UDP-N-acetylhexosamine pyrophosphorylase, UDP-N-acteylglucosamine pyrophosphorylase 1

Gene Symbol

UAP1

Additional UAP1 Products

Product Documents for UAP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for UAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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