UAP56 Antibody (2F5G7) - BSA Free

Novus Biologicals | Catalog # NBP2-52455

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F5G7

Format

BSA Free
View all UAP56 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human UAP56 (AA: 1-250) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UAP56 Antibody (2F5G7) - BSA Free

Western Blot: UAP56 Antibody (2F5G7)BSA Free [NBP2-52455]

Western Blot: UAP56 Antibody (2F5G7)BSA Free [NBP2-52455]

Western Blot: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis using DDX39B mAb against human DDX39B (AA: 1-250) recombinant protein. (Expected MW is 54.2 kDa)
Immunocytochemistry/ Immunofluorescence: UAP56 Antibody (2F5G7) - BSA Free [NBP2-52455]

Immunocytochemistry/ Immunofluorescence: UAP56 Antibody (2F5G7) - BSA Free [NBP2-52455]

Immunocytochemistry/Immunofluorescence: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis of HeLa cells using DDX39B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry: UAP56 Antibody (2F5G7) - BSA Free [NBP2-52455]

Immunohistochemistry: UAP56 Antibody (2F5G7) - BSA Free [NBP2-52455]

Immunohistochemistry: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis of paraffin-embedded HeLa tissues using DDX39B mouse mAb with DAB staining.
Western Blot: UAP56 Antibody (2F5G7)BSA Free [NBP2-52455]

Western Blot: UAP56 Antibody (2F5G7)BSA Free [NBP2-52455]

Western Blot: UAP56 Antibody (2F5G7) [NBP2-52455] - Western blot analysis using DDX39B mAb against HEK293 (1) and DDX39B (AA: 1-250)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: UAP56 Antibody (2F5G7)BSA Free [NBP2-52455]

Western Blot: UAP56 Antibody (2F5G7)BSA Free [NBP2-52455]

Western Blot: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis using DDX39B mouse mAb against HepG2 (1), HepG2 (2), K562 (3), Jurkat (4), NIH/3T3 (5), MCF-7 (6), Jurkat (7), and Hek293 (8) cell lysate.
Immunofluorescence: UAP56 Antibody (2F5G7) - BSA Free [NBP2-52455]

Immunofluorescence: UAP56 Antibody (2F5G7) - BSA Free [NBP2-52455]

Immunofluorescence: UAP56 Antibody (2F5G7) [NBP2-52455] - Analysis of HeLa cells using DDX39B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
ELISA: UAP56 Antibody (2F5G7) - BSA Free [NBP2-52455]

ELISA: UAP56 Antibody (2F5G7) - BSA Free [NBP2-52455]

ELISA: UAP56 Antibody (2F5G7) [NBP2-52455] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for UAP56 Antibody (2F5G7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UAP56

UAP56 encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described.

Alternate Names

56 kDa U2AF65-associated protein, ATP-dependent RNA helicase p47, DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B, DEAD box protein UAP56, EC 3.6.4.13, HLA-B associated transcript 1, HLA-B-associated transcript 1 protein, spliceosome RNA helicase BAT1, UAP56D6S81EBAT1nuclear RNA helicase (DEAD family)

Gene Symbol

DDX39B

Additional UAP56 Products

Product Documents for UAP56 Antibody (2F5G7) - BSA Free

Certificate of Analysis

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Product Specific Notices for UAP56 Antibody (2F5G7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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