UAP56 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-58594

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UAP56 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: MAENDVDNELLDYEDDEVETAAGGD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UAP56 Antibody - BSA Free

Western Blot: UAP56 Antibody [NBP2-58594]

Western Blot: UAP56 Antibody [NBP2-58594]

Western Blot: UAP56 Antibody [NBP2-58594] - Analysis in human cell lines HEK293 and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunocytochemistry/ Immunofluorescence: UAP56 Antibody [NBP2-58594]

Immunocytochemistry/ Immunofluorescence: UAP56 Antibody [NBP2-58594]

Immunocytochemistry/Immunofluorescence: UAP56 Antibody [NBP2-58594] - Staining of human cell line MCF7 shows localization to nuclear speckles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: UAP56 Antibody [NBP2-58594]

Immunohistochemistry-Paraffin: UAP56 Antibody [NBP2-58594]

Immunohistochemistry-Paraffin: UAP56 Antibody [NBP2-58594] - Staining in human epididymis and duodenum tissues using anti-DDX39B antibody. Corresponding DDX39B RNA-seq data are presented for the same tissues.
Western Blot: UAP56 Antibody [NBP2-58594]

Western Blot: UAP56 Antibody [NBP2-58594]

Western Blot: UAP56 Antibody [NBP2-58594] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunohistochemistry-Paraffin: UAP56 Antibody [NBP2-58594]

Immunohistochemistry-Paraffin: UAP56 Antibody [NBP2-58594]

Immunohistochemistry-Paraffin: UAP56 Antibody [NBP2-58594] - Staining of human duodenum shows low expression as expected.
Immunohistochemistry-Paraffin: UAP56 Antibody [NBP2-58594]

Immunohistochemistry-Paraffin: UAP56 Antibody [NBP2-58594]

Immunohistochemistry-Paraffin: UAP56 Antibody [NBP2-58594] - Staining of human epididymis shows high expression.

Applications for UAP56 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UAP56

UAP56 encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described.

Alternate Names

56 kDa U2AF65-associated protein, ATP-dependent RNA helicase p47, DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B, DEAD box protein UAP56, EC 3.6.4.13, HLA-B associated transcript 1, HLA-B-associated transcript 1 protein, spliceosome RNA helicase BAT1, UAP56D6S81EBAT1nuclear RNA helicase (DEAD family)

Gene Symbol

DDX39B

Additional UAP56 Products

Product Documents for UAP56 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for UAP56 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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