Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UBAP1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human UBAP1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for UBAP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: UBAP1 Antibody [NBP2-98559]

Immunohistochemistry-Paraffin: UBAP1 Antibody [NBP2-98559]

Immunohistochemistry-Paraffin: UBAP1 Antibody [NBP2-98559] - Immunochemical staining of human UBAP1 in human pancreas with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: UBAP1 Antibody [NBP2-98559]

Immunohistochemistry-Paraffin: UBAP1 Antibody [NBP2-98559]

Immunohistochemistry-Paraffin: UBAP1 Antibody [NBP2-98559] - Immunochemical staining of human UBAP1 in human large intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
UBAP1 Antibody

Western Blot: UBAP1 Antibody [NBP2-98559] -

Western Blot: UBAP1 Antibody [NBP2-98559] - - Anti-UBAP1 rabbit polyclonal antibody at 1:2000 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 60 kDa
UBAP1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: UBAP1 Antibody [NBP2-98559] -

Immunocytochemistry/Immunofluorescence: UBAP1 Antibody [NBP2-98559] - Immunofluorescence staining of UBAP1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98559 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for UBAP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBAP1

UBAP1 is a member of the UBA domain family, whose members include proteins having connections to ubiquitin and the ubiquitination pathway. The ubiquitin associated domain is thought to be a non-covalent ubiquitin binding domain consisting of a compact three helix bundle. This particular protein originates from a gene locus in a refined region on chromosome 9 undergoing loss of heterozygosity in nasopharyngeal carcinoma (NPC). Taking into account its cytogenetic location, this UBA domain family member is being studies as a putative target for mutation in nasopharyngeal carcinomas. [provided by RefSeq]

Alternate Names

MGC119669, MGC8710, NAG20, Nasopharyngeal carcinoma-associated gene 20 protein, UAP, UBAP, UBAP-1, ubiquitin associated protein, ubiquitin associated protein 1, ubiquitin-associated protein 1

Gene Symbol

UBAP1

Additional UBAP1 Products

Product Documents for UBAP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for UBAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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