UbcH10/UBE2C Antibody (1F5D3) - BSA Free

Novus Biologicals | Catalog # NBP2-52580

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1F5D3

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human UbcH10/UBE2C (AA: FULL(1-179)) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

19.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UbcH10/UBE2C Antibody (1F5D3) - BSA Free

Western Blot: UbcH10/UBE2C Antibody (1F5D3)BSA Free [NBP2-52580]

Western Blot: UbcH10/UBE2C Antibody (1F5D3)BSA Free [NBP2-52580]

Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mouse mAb against Hela (1), and Raji (2) cell lysate.
Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (1F5D3) - BSA Free [NBP2-52580]

Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (1F5D3) - BSA Free [NBP2-52580]

Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis of paraffin-embedded esophageal cancer tissues using UBE2C mouse mAb with DAB staining.
Western Blot: UbcH10/UBE2C Antibody (1F5D3)BSA Free [NBP2-52580]

Western Blot: UbcH10/UBE2C Antibody (1F5D3)BSA Free [NBP2-52580]

Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mAb against human UBE2C (AA: FULL(1-179)) recombinant protein. (Expected MW is 45.6 kDa)
Western Blot: UbcH10/UBE2C Antibody (1F5D3)BSA Free [NBP2-52580]

Western Blot: UbcH10/UBE2C Antibody (1F5D3)BSA Free [NBP2-52580]

Western Blot: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis using UBE2C mAb against HEK293 (1) and UBE2C (AA: FULL(1-179))-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (1F5D3) - BSA Free [NBP2-52580]

Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (1F5D3) - BSA Free [NBP2-52580]

Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Analysis of paraffin-embedded liver cancer tissues using UBE2C mouse mAb with DAB staining.
ELISA: UbcH10/UBE2C Antibody (1F5D3) - BSA Free [NBP2-52580]

ELISA: UbcH10/UBE2C Antibody (1F5D3) - BSA Free [NBP2-52580]

ELISA: UbcH10/UBE2C Antibody (1F5D3) [NBP2-52580] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for UbcH10/UBE2C Antibody (1F5D3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UbcH10/UBE2C

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Alternate Names

UBE2C

Gene Symbol

UBE2C

Additional UbcH10/UBE2C Products

Product Documents for UbcH10/UBE2C Antibody (1F5D3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for UbcH10/UBE2C Antibody (1F5D3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail