UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free

Name: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-20107
Price: 219 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-20107

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Mass Spectrometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SAIC-41A-1

Format

Azide and BSA Free

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Product Specifications

Immunogen

Peptide "GISAFPESDNLFK", derived from the Ubiquitin-conjugating enzyme E2C, conjugated to KLH.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free

Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] -

Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow Cytometry: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] -

Flow Cytometry: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-41A-1 (NBP3-20107, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] -

Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-41A-1 (NBP3-20107) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing NUCLEAR staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20107, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.