UbcH5a/UBE2D1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32734

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UbcH5a/UBE2D1 Primary Antibodies »

Product Specifications

Immunogen

Full length human UbcH5a/UBE2D1 Recombinant protein.

Reactivity Notes

Xenopus laevis (95%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UbcH5a/UBE2D1 Antibody - BSA Free

Western Blot: UbcH5a/UBE2D1 Antibody [NBP1-32734]

Western Blot: UbcH5a/UBE2D1 Antibody [NBP1-32734]

Western Blot: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: UbcH5a/UBE2D1 Antibody [NBP1-32734]

Immunocytochemistry/ Immunofluorescence: UbcH5a/UBE2D1 Antibody [NBP1-32734]

Immunocytochemistry/Immunofluorescence: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Analysis of paraformaldehyde-fixed A431, using UBE2D1 antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734]

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734]

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Mouse lung. UBE2D1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734]

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734]

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734] - TOV-112D xenograft, using UBE2D1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734]

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734]

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody [NBP1-32734] - Mouse prostate. UBE2D1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for UbcH5a/UBE2D1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UbcH5a/UBE2D1

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Alternate Names

E2(17)KB1, UBC4/5, UBC5A, UBE2D1

Gene Symbol

UBE2D1

Additional UbcH5a/UBE2D1 Products

Product Documents for UbcH5a/UBE2D1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UbcH5a/UBE2D1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail