UbcH5b/UBE2D2 Antibody (4A1) - Azide and BSA Free

Novus Biologicals | Catalog # H00007322-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4A1

Format

Azide and BSA Free
View all UbcH5b/UBE2D2 Primary Antibodies »

Product Specifications

Immunogen

UBE2D2 (NP_003330, 1 a.a. ~ 94 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWS

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

UBE2D2 (4A1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for UbcH5b/UBE2D2 Antibody (4A1) - Azide and BSA Free

Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02]

Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02]

Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - UBE2D2 monoclonal antibody (M02), clone 4A1. Analysis of UBE2D2 expression in Raw 264.7.
Immunohistochemistry-Paraffin: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02]

Immunohistochemistry-Paraffin: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02]

Immunohistochemistry-Paraffin: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - Analysis of monoclonal antibody to UBE2D2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02]

Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02]

Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - UBE2D2 monoclonal antibody (M02), clone 4A1. Analysis of UBE2D2 expression in PC-12.
Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02]

Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02]

Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - UBE2D2 monoclonal antibody (M02), clone 4A1 Analysis of UBE2D2 expression in NIH/3T3.
Sandwich ELISA: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - Detection limit for recombinant GST tagged UBE2D2 is approximately 3ng/ml as a capture antibody.

Applications for UbcH5b/UBE2D2 Antibody (4A1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UbcH5b/UBE2D2

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Alternate Names

PUBC1, UBC5B, UBE2D2

Entrez Gene IDs

7322 (Human)

Gene Symbol

UBE2D2

OMIM

602962 (Human)

UniProt

NP_003330 (Human)

Additional UbcH5b/UBE2D2 Products

Product Documents for UbcH5b/UBE2D2 Antibody (4A1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UbcH5b/UBE2D2 Antibody (4A1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for UbcH5b/UBE2D2 Antibody (4A1) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail